Generic placeholder image

Anti-Infective Agents


ISSN (Print): 2211-3525
ISSN (Online): 2211-3533

Research Article

Biological Activities of Papaya (Carica papaya L.) Seed, Rind and Pulp Oil Extracts

Author(s): Aynalem Getahun, Zekeria Yusuf* and Mulugeta Desta

Volume 21, Issue 4, 2023

Published on: 26 May, 2023

Article ID: e310323215317 Pages: 5

DOI: 10.2174/2211352521666230331095207

Price: $65


Background: Carica Papaya L. is widely cultivated for food and traditional medicine throughout the World due to its rich source of bioactive compounds in its fruit, leaves, latex and stem.

Objective: The aim of this work was to assess the physicochemical properties, and antioxidant and antimicrobial activities of oils extracted from papaya seeds, fruit pulp and rind using the solvent extraction method.

Methods: Hexane mediated extraction of oil was made. Then, physicochemical properties of the oil extracts were based on the determination of oil yield, specific gravity, acid value, free fatty acid and peroxide value, while the antioxidant activities were conducted based on ascorbic acid content, DPPH and hydrogen peroxide free radical scavenging activities. The antimicrobial experiment was conducted based on disc diffusion and broth dilution methods.

Results: The result of physicochemical properties of papaya seeds, pulp and rind oil extracts demonstrated significantly the highest oil yield (42.50%) and specific gravity (0.85) obtained for seed oil. Significantly the highest DPPH (19.35%), and hydrogen peroxide scavenging activities (32.45%) were recorded for seed oil extract. The strongest antibacterial activity with maximum zone of inhibition (18.50 mm), minimum inhibitory concentration MIC (0.03 μg/mL) and minimum bactericidal concentration MBC (0.05 μg/mL) were recorded for seed oil extract against S. pyogenes. However, the strongest antifungal activity with maximum zone of inhibition (16.75 mm), MIC (0.05 μg/mL) and MFC (0.10 μg/mL) against C. albicans was recorded for rind oil extract against C. albicans.

Conclusion: It can be concluded from the result of this study that seed oil was found to have antioxidant and antibacterial potential but fruit rind oil extract was the most effective antifungal in papaya.

Keywords: Antifungal activity, antibacterial potential, bactericidal concentration, free radical scavenging activity, Hexane mediated extraction, antioxidant potential.

Graphical Abstract
Srivastava, A.K.; Singh, V.K. Carica Papaya- A herbal medicine. Int. J. Res. Stud. Biosci., 2016, 4(11), 19-25.
Syed, H.M.; Kunte, S.P.; Jadhav, B.A.; Salve, R.V. Extraction and characterization of papaya seed oil. Elixir Agri., 2011, 37A, 4102-4105.
Adigwe, C.C.; Oremosu, A.A.; Gbotolorun, S.C.; Kuseiju, T.O.; Akang, E.N.; Noronha, C.C.; Okalanwon, A.O. Effects of the alcoholic root extract of the male Carica papaya on ovulation, gestation and fetal parameters in female Sprague- Dawley rats. J. Med. Plants Res., 2012, 6(16), 3093-3097.
Aravind, G.; Debjit, B.; Duraivel, S.; Harish, G. Traditional and medicinal uses of carica papaya. J. Med. Plants Stud., 2013, 1(1), 7-15.
Ayoola, P.B.; Adeyeye, A. Phytochemical and nutrient evaluation of Carica papaya (pawpaw) leaves. IJRRAS, 2010, 5(3), 325-328.
Imaga, N.A.; George, O.; Gbenle, V.I.; Okochi, S.A.; Tomi, D.; Bola, O.; Patience, N.; Dokai, M.O.; Alero, O.; Felix, C.E. Phytochemical and antioxidant nutrient constituents of Carica papaya and Parquetina nigrescens extracts. Sci. Res. Essays, 2010, 5(16), 2201-2205.
Jaiswal, P.; Kumar, P.; Singh, V.K.; Singh, D.K. Review article. Carica papaya Linn: A potential source for various health problems. J. Pharm. Res., 2010, 3(5), 998-1003.
Kermanshai, R.; McCarry, B.E.; Rosenfeld, J.; Summers, P.S.; Weretilnyk, E.A.; Sorger, G.J. Benzyl isothiocyanate is the chief or sole anthelmintic in papaya seed extracts. Phytochemistry, 2001, 57(3), 427-435.
[] [PMID: 11393524]
Kalou, G.B.; Kimbonguila, A.; Nzikou, J.M.; Ganongo, B.; Moutoula, F.E.; Akdowa, P.; Silou, T.; Desobry, S. Extraction and characteristics of seed oil from Papaya (Carica papaya) in Congo-Brazzaville. Asian J. Agri. Sci., 2011, 3(2), 132-137.
Kantha, LK; Mondal, P; Somnath, D; Jana, S; Aneela, S; Satyavathi, K Evaluation of anthelmintic activity of Carica papaya latex using Pheritima posthuma. 2012, 2(1), 10-12.
Saran, P.L.; Choudhary, R. Drug bioavailability and traditional medicaments of commercially available papaya: A review. Afr. J. Agric. Res., 2013, 8(25), 3216-3223.
Association of Official Analytical Chemists’ (AOAC). Official Methods of Analysis, 15th ed; Association of Official Analytical Chemists’: Washington, DC, USA, 1990.
Clinical and Laboratory Standards Institute (CLSI). Performance standards for antimicrobial disk susceptibility tests; approved standards, Eleventh Edition; Clinical and Laboratory Standards Institute: Wayne, PA, USA, 2012.
Hudzicki, J. Kirby-Bauer Disk Diffusion Susceptibility Test Protocol; American Society for Microbiology: Washington, D.C., USA, 2009, p. 23.
SAS Institute. SAS enterprise guide, Version 9.2; SAS Inst.: Cary, NC, USA, 2011.
Syed, H.M.; Kunte, S.P.; Jadhav, B.A.; Salve, R.V. Extraction and characterization of papaya seed oil. Int. J. Appl. Phys. Bio-Chem. Res., 2012, 2(1), 33-43.
Muhamad, S.A.S.; Jamilah, B.; Russly, A.R.; Faridah, A. The antibacterial activities and chemical composition of extracts from Carica papaya cv. Sekaki/Hong Kong seed. Int. Food Res. J., 2017, 24(2), 810-818.

Rights & Permissions Print Cite
© 2024 Bentham Science Publishers | Privacy Policy