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Current Pharmaceutical Biotechnology


ISSN (Print): 1389-2010
ISSN (Online): 1873-4316

Research Article

The Phytochemical and Pharmacological Properties of Citrus sinensis ‘Maltese Half-blood’ Essential Oil Peels Extracted and Optimized by Response-surface Methodology

Author(s): Imen Kallel*, Nidhal Tarhouni, Asma Elaguel, Saoussen Mekrazi, Abdelmajid Khabir, Bilel Hadrich and Ahmed Bayoudh

Volume 24, Issue 15, 2023

Published on: 03 May, 2023

Page: [1938 - 1951] Pages: 14

DOI: 10.2174/1389201024666230405120520

Price: $65


Background: Citrus fruits have been a valuable economic crop for thousands of years. Furthermore, citrus essential oils are significant in the perfume, food, and beverage sectors, as well as aromatherapy and medical medicines.

Aims: The present study aims to evaluate the phytochemical and pharmacological potentials of the optimized Citrus sinensis ‘Maltese half-blood’ essential oils peels (CsEO) extraction yields using Response-Surface Methodology (RSM).

Objective: There have been few investigations on Citrus sinensis ‘Maltese half-blood’ essential oil.

Methods: Citrus sinensis ‘Maltese half-blood’ essential oil peels (CsEO) extraction yields were performed by hydro-distillation and optimized by using Response-Surface Methodology (RSM). The oils were analysed by GC-MS. Different chemical tests were used to evaluate antioxidant activities. The healing potential was evaluated using models’ wounds on Wistar rats.

Results: The RSM optimization demonstrated the highest yield of CsEO of 6.89 g/100 g d.b. All three tested factors significantly influenced the CsEO extraction yield: washing saline solution concentration, washings number, and drying percentage of peels. Significant antioxidant activities were noted in CsEO: the DPPH assay reported an IC50 of 0.225 ± 0.014 mL/mg, the FRAP assay showed an IC50 of 0.235 ± 0.001, and the NO assay was an IC50 in order of 0.259 ± 0.019. CsEO was not genotoxic and considerably decreased the levels of DNA lesions induced by oxidants. Also, applying a cream with CsEO on wounds promotes significantly rapid wound healing.

Conclusion: CsEO could be considered a rich natural source of antioxidants and bio-compounds to accelerate wound healing. It can be used in pharmaceutical sectors as an alternative to synthetic chemicals.

Keywords: Essential oils, RSM, Citrus sinensis, antioxidant activity, DNA damage protection, wound healing.

Graphical Abstract
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