Generic placeholder image

Current Drug Discovery Technologies

Editor-in-Chief

ISSN (Print): 1570-1638
ISSN (Online): 1875-6220

Research Article

Biosynthesis of Zinc Oxide Nanoparticles by Lactobacillus spp. and Investigation of their Antimicrobial Effect

Author(s): Saeideh Morovaty Sharifabady, Pegah Shakib, Behin Omidi and Mohammad Reza Zolfaghari*

Volume 20, Issue 5, 2023

Published on: 09 June, 2023

Article ID: e010523216388 Pages: 9

DOI: 10.2174/1570163820666230501152951

Price: $65

Abstract

Background: Nanoparticle biology is preferable to other common methods due to its economic efficiency and compatibility with the environment. On the other hand, the prevalence of drug-resistant bacterial strains is expanding and it is necessary to use alternative antibiotic compounds to deal with them. The aim of the present study was the biosynthesis of zinc oxide nanoparticles(ZnO NPs) by Lactobacillus spp. and their antimicrobial effect.

Methods: In this study, after the biosynthesis of ZnO NPs by Lactobacillus spp, Characterization of Nanoparticulation Was performed by UV–Vis, XRD, and Scanning Electron Microscopy (SEM). Additionally, Lactobacillus spp. - ZnO NPs were assessed for their antimicrobial properties.

Results: UV-visible spectroscopy confirmed the Lactobacillus spp. - ZnO NPs absorbed UV in the region of 300-400 nm. XRD analysis showed the presence of zinc metal in nanoparticles. SEM revealed that Lactobacillus plantarum - ZnO NPs were smaller than the others. Staphylococcus aureus showed the largest non-growth halo diameter against ZnO NPs synthesized by L. plantarum ATCC 8014 (3.7 mm). E. coli had the largest growth halo diameter against ZnO NPs synthesized by L. casei (3 mm) and L. plantarum (2.9 mm). The MIC values of ZnO NPs synthesized by L. plantarum ATCC 8014, L.casei ATCC 39392, L. fermenyum ATCC 9338, L. acidophilus ATCC 4356 were 2,8,8 and 4 μg/mL for Staphylococcus aureus. The MIC values of ZnO NPs synthesized by L. plantarum ATCC 8014, L. casei ATCC 39392, L. fermenyum ATCC 9338, L. acidophilus ATCC 4356 were 2, 4, 4, and 4 μg/ml for E. coli. The lowest MICs were 2 μg/ml for E. coli and S. aureus related to ZnO NPs synthesized by L. plantarum ATCC 8014. MIC and MBC values were equivalent to each other.

Conclusion: The results of this research show that ZnO NPs synthesized by L. plantarum ATCC 8014 have more antimicrobial effects than other ZnO NPs used. Therefore, the ZnO NPs made with Lactobacillus plantarum ATCC 8014 have the potential to kill bacteria and can be considered a candidate for antibiotic replacement.

Keywords: Biosynthesis, Zinc oxide (ZnO NPs), Lactobacillus spp, Antimicrobial properties, nanoparticles, XRD.

Graphical Abstract
[1]
Whatmore RW. Nanotechnology-What is it? Should we be worried? Occup Med (Lond) 2006; 56(5): 295-9.
[http://dx.doi.org/10.1093/occmed/kql050] [PMID: 16868126]
[2]
Bala N, Saha S, Chakraborty M, et al. Green synthesis of zinc oxide nanoparticles using Hibiscus subdariffa leaf extract: effect of temperature on synthesis, anti-bacterial activity and anti-diabetic activity. RSC Advances 2015; 5(7): 4993-5003.
[http://dx.doi.org/10.1039/C4RA12784F]
[3]
Mandal S, Phadtare S, Sastry M. Interfacing biology with nanoparticles. Curr Appl Phys 2005; 5(2): 118-27.
[http://dx.doi.org/10.1016/j.cap.2004.06.006]
[4]
Ealia SAM, Saravanakumar M. A review on the classification, characterisation, synthesis of nanoparticles and their application. IOP Conf Ser: Mater Sci Eng 2017; 263: 032019.
[5]
Jiang J, Pi J. The advancing of zinc oxide nanoparticles for biomedical applications. Bioinorg Chem Appl 2018; 2018: 1062562.
[6]
Sirelkhatim A, Mahmud S, Seeni A, et al. Review on zinc oxide nanoparticles: Antibacterial activity and toxicity mechanism. Micro Nano Lett 2015; 7(3): 219-42.
[7]
Miri A, Mahdinejad N, Ebrahimy O, Khatami M, Sarani MJMS. Zinc oxide nanoparticles: Biosynthesis, characterization, antifungal and cytotoxic activity. Mater Sci Eng C 2019; 104: 109981.
[8]
Zare E, Pourseyedi S, Khatami M, Darezereshki E. Simple biosynthesis of zinc oxide nanoparticles using nature’s source, and it’s in vitro bio-activity. J Mol Struct 2017; 1146: 96-103.
[9]
Mohd MH, Mohamad R, Abdul Rahman NA, Zaidan UH. Microbial synthesis of zinc oxide nanoparticles and their potential application as an antimicrobial agent and a feed supplement in animal industry: A review. J Anim Sci Biotechnol 2019; 10(1): 1-22.
[10]
Mirzaei H, Darroudi MJCI. Zinc oxide nanoparticles: Biological synthesis and biomedical applications. Ceram Int 2017; 43(1): 907-14.
[11]
Agarwal H, Menon S, Kumar SV, Rajeshkumar S. Mechanistic study on antibacterial action of zinc oxide nanoparticles synthesized using green route. Chem Biol Interact 2018; 286: 60-70.
[12]
Sharma N, Kumar J, Thakur S, Sharma S, Shrivastava VJDIT. Antibacterial study of silver doped zinc oxide nanoparticles against Staphylococcus aureus and Bacillus subtilis. Drug Invent Today 2013; 5(1): 50-4.
[13]
Kadiyala U, Turali-Emre ES, Bahng JH, Kotov NA, VanEpps JSJN. Unexpected insights into antibacterial activity of zinc oxide nanoparticles against methicillin resistant Staphylococcus aureus (MRSA). Nanoscale 2018; 10(10): 4927-39.
[14]
Akbar A, Sadiq MB, Ali I, et al. Synthesis and antimicrobial activity of zinc oxide nanoparticles against foodborne pathogens Salmonella typhimurium and Staphylococcus aureus. Biocatal Agric Biotechnol 2019; 17: 36-42.
[15]
Khosravi-Darani K, Gomes da Cruz A, Shamloo E, Abdimoghaddam Z, Mozafari M. Green synthesis of metallic nanoparticles using algae and microalgae. Lett Appl Microbiol 2019; 8: 666-70.
[16]
Tayel AA. Antibacterial action of zinc oxide nanoparticles against foodborne pathogens. J Food Saf 2011; 31(2): 211-8.
[17]
Tayel AA, Moussa S, Opwis K, Knittel D, Schollmeyer E, Nickisch-Hartfiel AJ. Inhibition of microbial pathogens by fungal chitosan. Int J Biol Macromol 2010; 47(1): 10-4.
[18]
Khajuria AK, Kumari M, Kandwal A, Singh A, Bisht NJB. Biofabrication of zinc oxide nanoparticles from two different zinc sources and their antimicrobial activity. Bionanosci 2021; 11(3): 793-809.
[19]
Varadavenkatesan T, Lyubchik E, Pai S, Pugazhendhi A, Vinayagam R. Photocatalytic degradation of Rhodamine B by zinc oxide nanoparticles synthesized using the leaf extract of Cyanometra ramiflora. J Photochem Photobiol B 2019; 199: 111621.
[20]
Ahmed T, Wu Z, Jiang H, et al. Bioinspired green synthesis of zinc oxide nanoparticles from a native Cyanometra ramiflora strain RNT6: Characterization and antibacterial activity against rice panicle blight pathogens Burkholderia glumae and Burkholderia glumae. Nanomaterials (Basel) 2021; 11(4): 884.
[21]
Mohd Yusof H, Rahman A, Mohamad R, Zaidan UH. Samsudin AAJSr. Biosynthesis of zinc oxide nanoparticles by cell-biomass and supernatant of Lactobacillus plantarume TA4 and its antibacterial and biocompatibility properties. Sci Rep 2020; 10(1): 1-13.
[22]
Mohamed AA, Fouda A, Abdel-Rahman MA, et al. Fungal strain impacts the shape, bioactivity and multifunctional properties of green synthesized zinc oxide nanoparticles. Biocatal Agric Biotechnol 2019; 19: 101103.
[23]
Selvarajan E, Mohanasrinivasan VJML. Biosynthesis and characterization of ZnO nanoparticles using Lactobacillus plantarume VITES07. Mater Lett 2013; 112: 180-2.
[24]
Siddiqui VU, Ansari A, Ansari MT, et al. Optimization of facile synthesized ZnO/CuO nanophotocatalyst for organic dye degradation by visible light irradiation using response surface methodology. Catalysts 2021; 11(12): 1509.
[25]
Suba S, Vijayakumar S, Vidhya E, Punitha V, Nilavukkarasi MJSI. Microbial mediated synthesis of ZnO nanoparticles derived from Lactobacillus spp: Characterizations, antimicrobial and biocompatibility efficiencies Sensors Int 2021; 2: 100104.
[26]
El-Sayed HS, El-Sayed SM, Youssef AMJFC. Novel approach for biosynthesizing of zinc oxide nanoparticles using Lactobacillus gasseri and their influence on microbiological, chemical, sensory properties of integrated yogurt. Food Chem 2021; 365: 130513.
[27]
Salman JAS, Kadhim AA, Haider AJJJGPT. Biosynthesis, characterization and antibacterial effect of ZnO nanoparticles synthesized by Lactobacillus Spp. J Glob Pharma Technol 2018; 10(03): 348-55.
[28]
Prakashkumar N, Pugazhendhi A, Brindhadevi K, Garalleh HA, Garaleh M, Suganthy NJER. Comparative study of zinc oxide nanoparticles synthesized through biogenic and chemical route with reference to antibacterial, antibiofilm and anticancer activities. Environ Res 2023; 220: 115136.

Rights & Permissions Print Export Cite as
© 2023 Bentham Science Publishers | Privacy Policy