Introduction: Curcumin is a naturally occurring compound that has antioxidant properties, acts as a hepatoprotective, and lowers lipid peroxidation. However, curcumin's low solubility and bioavailability are its primary drawbacks and prevent its use as a therapeutic agent. In this study, curcumin nanoparticles will be created using the ultrasonic-assisted extraction method, and their effectiveness against paracetamol-induced changes in ALT, AST, SOD, MDA, and TNF-α will be compared to that of pure curcumin.
Purpose: This study aimed to determine the hepatoprotective effect of curcumin nanoparticles in paracetamol- induced rats as a model for liver injury.
Methods: Thirty-six male Wistar rats, aged 6 to 8 weeks, with a minimum weight of 120 grams, were used in an experimental laboratory investigation with a post-test-only group design. Rats in each group received 100 mg/kgBW pure curcumin, 100 mg/kgBW curcumin nanoparticles, and 50 mg/kgBW curcumin nanoparticles for 7 days before paracetamol induction. On day 8, 300 mg/kgBW of paracetamol was intraperitoneally injected to cause liver damage. One of the groups received NAC as an antidote 10 hours after paracetamol induction. Detection of ALT and AST using a Chemistry Analyzer. ELISA approach for the detection of SOD, MDA, and TNF-α. The Roenigk score was calculated by two examiners after the liver histopathology preparations were stained using the Hematoxylin-Eosin method. Post hoc analyses were performed after the One Way Annova and Kruskal Wallis tests to examine the data.
Results: According to PSA results, the smallest formula that formed curcumin nanoparticles (10.2 nm) was 8 g of curcumin formula mixed with a mixture of Tween 20 4.5 ml, Kolliphor EL 1.5 ml, Propylene Glycol 1.5 ml, and Capryol 90 1 ml for 21 minutes using an ultrasonic process. MDA and TNF-α levels, as well as the liver's histological Roenigk score, were significantly lower in the 100 mg/kgBB pure curcumin group (C100) when compared to the model group (model). The levels of AST, MDA, TNF-α, and the liver histopathology score were significantly lower in the 100 mg/kgBB (NC100) and 50 mg/kgBB (NC50) curcumin nanoparticle groups compared to the model group (model) and pure curcumin group (C100) (p< 0.05).
Conclusion: Curcumin nanoparticles showed better hepatoprotective ability than pure curcumin.
[http://dx.doi.org/10.1016/j.taap.2012.08.015] [PMID: 22980195]
[http://dx.doi.org/10.3390/molecules26247658] [PMID: 34946740]
[http://dx.doi.org/10.1016/j.fct.2010.08.034] [PMID: 20804811]
[http://dx.doi.org/10.1016/j.brainresbull.2015.11.019] [PMID: 26639783]
[http://dx.doi.org/10.1002/mnfr.202100613] [PMID: 34665507]
[http://dx.doi.org/10.2174/2211738510666220422135519] [PMID: 35466890]
[http://dx.doi.org/10.2174/2211738508666200421113215] [PMID: 32316907]
[http://dx.doi.org/10.2174/2211738509666211209164105] [PMID: 34886791]
[http://dx.doi.org/10.1016/j.ejpb.2010.07.011] [PMID: 20659556]
[http://dx.doi.org/10.1186/s12986-019-0331-1] [PMID: 30705687]
[http://dx.doi.org/10.3390/molecules24152802] [PMID: 31374848]
[http://dx.doi.org/10.1002/ptr.7375] [PMID: 35040210]
[http://dx.doi.org/10.1016/j.brainresbull.2018.01.006] [PMID: 29325994]