Follicular Helper T Cells in Peyer's Patches and Galactose- Deficient Iga1 Contribute to Iga Nephropathy

Title:Follicular Helper T Cells in Peyer's Patches and Galactose- Deficient Iga1 Contribute to Iga Nephropathy

Volume: 24 Issue: 8

Author(s): Yuye Huang, Xunling Sun, Guoming Nie, Hongtao Xu and Minshu Zou*

Affiliation:

• Department of Pediatrics, General Hospital of Central Theater Command, No. 627, Wuluo Road, Wuhan 430030, Hubei Province, P.R. China

Keywords: IgA nephropathy, Peyer’s patches, T follicular helper cell, CD4 T Cells, Interleukin-21, Galactosedeficient IgA1.

Abstract:

Background: Common primary glomerulonephritis with aberrant mucosal immunity is IgA nephropathy (IgAN). T follicular helper (TFH) cells are essential in regulating B cell differentiation. Peyer’s patches (PPs) are the main site where IgA+ plasmablasts differentiate.

Objective: Our study aimed to investigate the TFH cell's potential contribution to the etiology of IgA nephropathy.

Methods: In PPs from IgAN mouse models, the ratio of the TFH cell, B220+IgA+, B220+IgM+, and B220-IgA+ lymphocytes were assessed. Then, we used Western blot to assess the expression of Bcl-6, Blimp- 1, and IL-21 proteins in PPs and used RTPCR to assess the expression of IL-21 and TGF-β1 mRNA. TFH cells coculture with spleen cells to measure the degree of IL-21 and the ratio of activation marker CD69 on the TFH cells. Naive B cells (CD27-IgD+) from children suffering from IgAN were cultured with TFH cell-related cytokines. The supernatant was detected to assess the excretion of galactose-deficient IgA1 (Gd-IgA1).

Results: IgAN mice developed noticeably increased degrees of IL-21 and CD69 on TFH cells than controls did, as well as higher percentages of B220+IgA+, B220+IgM+, B220+IgA+, TGF- β1, and IL-21 mRNA and Bcl-6, IL-21 proteins in PPs. The Gd-IgA1 level in the supernatant and IgAN- positive children's serum were noticeably higher than those of the healthy controls (P < 0.05). PPs provide the microenvironment to induce the production of IgA-secreting plasmablasts.

Conclusion: TFH cells may be a key moderator to induce B cell differentiation into IgAsecreting plasmablasts and produce Gd-IgA1, which plays a significant part in IgAN’s pathogenesis. It could be a new therapeutic target in the future.

Cite this article as:

Huang Yuye, Sun Xunling, Nie Guoming, Xu Hongtao and Zou Minshu*, Follicular Helper T Cells in Peyer's Patches and Galactose- Deficient Iga1 Contribute to Iga Nephropathy, Current Molecular Medicine 2024; 24 (8) . https://dx.doi.org/10.2174/1566524023666230720112215