Cholecalciferol Exhibits no Antibacterial Effect on Staphylococcus aureus and Escherichia coli: An in vitro Study

Title:Cholecalciferol Exhibits no Antibacterial Effect on Staphylococcus aureus and Escherichia coli: An in vitro Study

Volume: 19 Issue: 4

Author(s): Shilia Jacob Kurian, Avirup Biswas, Shrivathsa Kulavalli, Jesil Mathew Aranjani, Amrita Pattanaik, Murali Munisamy, Kavitha Saravu, Gabriel Sunil Rodrigues and Sonal Sekhar Miraj*

Affiliation:

• Department of Pharmacy Practice, Manipal College of Pharmaceutical Sciences, Manipal Academy of High Education, Manipal, Karnataka, India
• Manipal Centre for Infectious Diseases, Prasanna School of Public Health, Manipal Academy of Higher Education, Manipal, Karnataka, India

Keywords: Cholecalciferol, diabetic foot ulcers, Escherichia coli, Staphylococcus aureus, vitamin D3, antibacterial effect.

Abstract:

Background: The pleiotropic effect of cholecalciferol (vitamin D₃) has gained significant momentum and has been explored widely.

Objectives: The study aimed to investigate the antimicrobial effect of cholecalciferol against S. aureus and E. coli.

Methods: An in vitro study was performed for the antimicrobial effect of cholecalciferol against S. aureus and E. coli. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined following the broth microdilution method.

Results: The MIC value of cholecalciferol against both S. aureus and E. coli was 0.312 mg/ml, and the MBC for both organisms was 1.25 mg/ml. However, we also observed a significant antimicrobial effect in the dimethyl sulfoxide (DMSO) control at 12.5% (v/v). Therefore, the observed antimicrobial effect may be attributed to DMSO, indicating cholecalciferol does not directly inhibit S. aureus and E. coli.

Conclusion: This study indicates that cholecalciferol does not directly inhibit S. aureus and E. coli. Hence, we suggest exploring the antibacterial properties of other vitamin D analogs, such as calcitriol or its synergetic effect with other antimicrobial agents.

Cite this article as:

Kurian Jacob Shilia, Biswas Avirup, Kulavalli Shrivathsa, Aranjani Mathew Jesil, Pattanaik Amrita, Munisamy Murali, Saravu Kavitha, Rodrigues Sunil Gabriel and Miraj Sekhar Sonal*, Cholecalciferol Exhibits no Antibacterial Effect on Staphylococcus aureus and Escherichia coli: An in vitro Study, Recent Advances in Anti-Infective Drug Discovery 2024; 19 (4) . https://dx.doi.org/10.2174/0127724344277290231211051800