Abstract
Biosensors are available and applicable for detection and characterization of specific inhibitors of many enzymes. In this review, biosensors based on fixed acetylcholinesterase (AChE) or butyrylcholinesterase (BChE) are presented. Inhibition of selected enzymes by various compounds, such as organophosphorus and carbamate pesticides, nerve agents (e.g. sarin or VX), and other natural toxins (e.g. aflatoxins), was employed to develop specific assays using biosensors only. Biosensor technology brings potential miniaturization and portability, when it is compared to standard methods. Construction of biosensors based on cholinesterases became a more important issue within the last decades. Novel approach with recombinant proteins, microelectrodes and immobilization protocol related to nanotechnologies opened new insight to the cholinesterase based biosensor construction and its perspective via routine assays. This review is focused on novel trends within such biosensors as a result of the known platform.
Keywords: Biosensor, acetylcholinesterase, butyrylcholinesterase, inhibitor, organophosphate, pharmacology
Current Medicinal Chemistry
Title: Progress of Biosensors Based on Cholinesterase Inhibition
Volume: 16 Issue: 14
Author(s): Miroslav Pohanka, Kamil Musilek and Kamil Kuca
Affiliation:
Keywords: Biosensor, acetylcholinesterase, butyrylcholinesterase, inhibitor, organophosphate, pharmacology
Abstract: Biosensors are available and applicable for detection and characterization of specific inhibitors of many enzymes. In this review, biosensors based on fixed acetylcholinesterase (AChE) or butyrylcholinesterase (BChE) are presented. Inhibition of selected enzymes by various compounds, such as organophosphorus and carbamate pesticides, nerve agents (e.g. sarin or VX), and other natural toxins (e.g. aflatoxins), was employed to develop specific assays using biosensors only. Biosensor technology brings potential miniaturization and portability, when it is compared to standard methods. Construction of biosensors based on cholinesterases became a more important issue within the last decades. Novel approach with recombinant proteins, microelectrodes and immobilization protocol related to nanotechnologies opened new insight to the cholinesterase based biosensor construction and its perspective via routine assays. This review is focused on novel trends within such biosensors as a result of the known platform.
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Cite this article as:
Pohanka Miroslav, Musilek Kamil and Kuca Kamil, Progress of Biosensors Based on Cholinesterase Inhibition, Current Medicinal Chemistry 2009; 16 (14) . https://dx.doi.org/10.2174/092986709788186129
DOI https://dx.doi.org/10.2174/092986709788186129 |
Print ISSN 0929-8673 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-533X |
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