Abstract
The cysteine-rich N-terminal domain of the micronemal adhesive protein MIC1 (MIC1-NT) from Toxoplasma gondii was cloned, expressed in Escherichia coli and purified. MIC1-NT is amenable to structural studies as shown by preliminary NMR and X-ray analysis. Positive results with two further micronemal proteins indicate that our strategy has wider application.
Keywords: toxoplasma gondii, micronemal proteins, cysteine-rich proteins, nmr, x-ray crystallography
Protein & Peptide Letters
Title: High-Level Bacterial Expression and Purification of Apicomplexan Micronemal Proteins for Structural Studies
Volume: 14 Issue: 5
Author(s): S. Saouros, T. M.A. Blumenschein, K. Sawmynaden, J. Marchant, T. Koutroukides, B. Liu, P. Simpson, E. P. Carpenter and S. J. Matthews
Affiliation:
Keywords: toxoplasma gondii, micronemal proteins, cysteine-rich proteins, nmr, x-ray crystallography
Abstract: The cysteine-rich N-terminal domain of the micronemal adhesive protein MIC1 (MIC1-NT) from Toxoplasma gondii was cloned, expressed in Escherichia coli and purified. MIC1-NT is amenable to structural studies as shown by preliminary NMR and X-ray analysis. Positive results with two further micronemal proteins indicate that our strategy has wider application.
Export Options
About this article
Cite this article as:
Saouros S., Blumenschein M.A. T., Sawmynaden K., Marchant J., Koutroukides T., Liu B., Simpson P., Carpenter P. E. and Matthews J. S., High-Level Bacterial Expression and Purification of Apicomplexan Micronemal Proteins for Structural Studies, Protein & Peptide Letters 2007; 14 (5) . https://dx.doi.org/10.2174/092986607780782876
DOI https://dx.doi.org/10.2174/092986607780782876 |
Print ISSN 0929-8665 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5305 |
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
Related Articles
-
Neuroinflammation and Immunity: A New Pharmacological Target in Depression
CNS & Neurological Disorders - Drug Targets Targeting MDM2 and MDMX in Retinoblastoma
Current Cancer Drug Targets Thiopurine Immunomodulators in Ulcerative Colitis: Moving Forward with Current Evidence
Current Drug Targets Platelet Biomarkers in Tumor Growth
Current Proteomics Transmembrane Phosphatases and Cancer Development, the Role of Protein Tyrosine Phosphatase-kappa (PTPκ) and Protein Tyrosine Phosphatase-mu (PTPμ)
Current Signal Transduction Therapy Current Drug Nano-targeting Strategies for Improvement in the Diagnosis and Treatment of Prevalent Pathologies such as Cardiovascular and Renal Diseases
Current Drug Targets Breast Cancer Stem Cells and Intrinsic Subtypes: Controversies Rage On
Current Stem Cell Research & Therapy Mitochondrial Dysfunction and Targeted Drugs: A Focus on Diabetes
Current Pharmaceutical Design Understanding the Use of Immunosuppressive Agents in the Clinical Management of IBD
Current Drug Targets High Yield Immobilization and Stabilization of Oxidoreductases Using Magnetic Nanosupports and Their Potential Applications: An Update
Current Catalysis The Potential of Natural Products as Effective Treatments for Allergic Inflammation: Implications for Allergic Rhinitis
Current Topics in Medicinal Chemistry Candidiasis: A Fungal Infection- Current Challenges and Progress in Prevention and Treatment
Infectious Disorders - Drug Targets EGFR(s) in Aging and Carcinogenesis of the Gastrointestinal Tract
Current Protein & Peptide Science Endothelin Receptors in Gastrointestinal Smooth Muscle
Current Protein & Peptide Science Genome and Transcriptome Analysis of Neuroblastoma Advanced Diagnosis from Innovative Therapies
Current Pharmaceutical Design miR-629-3p Level Significantly Predicts Prognosis in Glioblastoma Patients Treated with Temozolomide Chemotherapy
Current Signal Transduction Therapy Editorial [Hot Topic: Targeting Mast Cells and Basophils in Allergy and Beyond: Emerging Concepts (Executive Guest Editor: Petr Heneberg)]
Current Pharmaceutical Design Anti-Angiogenic Therapy as a Cancer Treatment Paradigm
Current Medicinal Chemistry - Anti-Cancer Agents Vasopressin in Health and Disease with a Focus on Affective Disorders
Central Nervous System Agents in Medicinal Chemistry Ontogenetic Variation of the Human Genome
Current Genomics