Abstract
β-Alanine synthase (EC 3.5.1.6) catalyzes the conversion of N-carbamyl-β-alanine to β-alanine, ammonia and CO2. The enzyme has been purified to apparent homogeneity from calf liver. The molecular size, pH optimum and substrate specificity have been determined. Sequence alignment of β-alanine synthases with N-carbamyl-D-amino acid amidohydrolase from Agrobacter sp. revealed the conservation of a catalytically important triad Glu-Lys-Cys, most likely involved in the breakdown of N-carbamyl-β-alanine.
Keywords: balanine, balanine synthases, pyrimidine degradation, neurotransmitter
Protein & Peptide Letters
Title: Purification and Properties of β-Alanine Synthase from Calf Liver
Volume: 12 Issue: 1
Author(s): Gunter Waldmann, Paul F. Cook and Klaus D. Schnackerz
Affiliation:
Keywords: balanine, balanine synthases, pyrimidine degradation, neurotransmitter
Abstract: β-Alanine synthase (EC 3.5.1.6) catalyzes the conversion of N-carbamyl-β-alanine to β-alanine, ammonia and CO2. The enzyme has been purified to apparent homogeneity from calf liver. The molecular size, pH optimum and substrate specificity have been determined. Sequence alignment of β-alanine synthases with N-carbamyl-D-amino acid amidohydrolase from Agrobacter sp. revealed the conservation of a catalytically important triad Glu-Lys-Cys, most likely involved in the breakdown of N-carbamyl-β-alanine.
Export Options
About this article
Cite this article as:
Waldmann Gunter, Cook F. Paul and Schnackerz D. Klaus, Purification and Properties of β-Alanine Synthase from Calf Liver, Protein & Peptide Letters 2005; 12 (1) . https://dx.doi.org/10.2174/0929866053405904
DOI https://dx.doi.org/10.2174/0929866053405904 |
Print ISSN 0929-8665 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5305 |
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
Related Articles
-
Actions of Rho-Kinase Inhibitors in Cardiovascular Diseases
Current Enzyme Inhibition Adolescents’ Rights: Better for Promoting Individual Rights or Public Health?
Adolescent Psychiatry Inhibition of Raf Kinase in the Treatment of Acute Myeloid Leukemia
Current Pharmaceutical Design Sirtuins Family- Recent Development as a Drug Target for Aging, Metabolism, and Age Related Diseases
Current Drug Targets Implication of Possible Therapies Targeted for the Tachykinergic System with the Biology of Neurokinin Receptors and Emerging Related Proteins
Recent Patents on CNS Drug Discovery (Discontinued) Protein Kinases and their Modulation in the Central Nervous System
Current Medicinal Chemistry - Central Nervous System Agents Clozapine Safety, 35 Years Later
Current Drug Safety Diffusion Tensor Imaging to Determine Effects of Antidementive Treatment on Cerebral Structural Connectivity in Alzheimer’s Disease
Current Pharmaceutical Design Risperidone Microemulsion for Transnasal Delivery: Pharmacodynamic and Pharmacokinetic Evaluation
Pharmaceutical Nanotechnology Protocatechuic Acid and Human Disease Prevention: Biological Activities and Molecular Mechanisms
Current Medicinal Chemistry The Molecular Basis of Memantine Action in Alzheimers Disease and Other Neurologic Disorders: Low-affinity, Uncompetitive Antagonism
Current Alzheimer Research Myalgic Encephalomyelitis: Symptoms and Biomarkers
Current Neuropharmacology Allosteric Targeting of Aurora A Kinase Using Small Molecules: A Step Forward Towards Next Generation Medicines?
Current Medicinal Chemistry Anti-NMDA Receptor Encephalitis in Psychiatry
Current Psychiatry Reviews Age-Related Changes in the Reliability of Antioxidant Enzyme Defense in Monkeys with Different Types of Adaptive Behavior
Current Aging Science Pathophysiology and Prevention of Bronchopulmonary Dysplasia
Current Pediatric Reviews A Study of Extrapyramidal Manifestations Accompanying Decompensated Viral Hepatic Cirrhosis Patients
Reviews on Recent Clinical Trials Functional and Molecular Ultrasound Imaging: Concepts and Contrast Agents
Current Medicinal Chemistry Delayed Cerebral Ischemia after Subarachnoid Hemorrhage: From Vascular Spasm to Cortical Spreading Depolarizations
Current Neurovascular Research Pulmonary Defence Mechanisms
Current Respiratory Medicine Reviews