Abstract
The recombinant minichaperone sht GroEL191-345 was cultivated in a 3.7 L stirred bioreactor with the high yield of 216.2 mg / L broth. In the refolding of recombinant human interferon gamma (rhuIFN-γ) inclusion bodies, more than 2-3 fold enhancement in protein mass recovery and total activity were observed in the presence of free or immobilized minichaperone to the refolding buffer.
Keywords: minichaperone, sht groel191-345, cultivation, purification, refolding, recombinant human interferon gamma
Protein & Peptide Letters
Title: Production of Minichaperone (sht GroEL191-345) and Its Function in the Refolding of Recombinant Human Interferon Gamma
Volume: 12 Issue: 1
Author(s): Yi-Xin Guan, Zheng-Zheng Fei, Man Luo, Shan-Jing Yao and Man-Gi Cho
Affiliation:
Keywords: minichaperone, sht groel191-345, cultivation, purification, refolding, recombinant human interferon gamma
Abstract: The recombinant minichaperone sht GroEL191-345 was cultivated in a 3.7 L stirred bioreactor with the high yield of 216.2 mg / L broth. In the refolding of recombinant human interferon gamma (rhuIFN-γ) inclusion bodies, more than 2-3 fold enhancement in protein mass recovery and total activity were observed in the presence of free or immobilized minichaperone to the refolding buffer.
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Cite this article as:
Guan Yi-Xin, Fei Zheng-Zheng, Luo Man, Yao Shan-Jing and Cho Man-Gi, Production of Minichaperone (sht GroEL191-345) and Its Function in the Refolding of Recombinant Human Interferon Gamma, Protein & Peptide Letters 2005; 12 (1) . https://dx.doi.org/10.2174/0929866053405995
DOI https://dx.doi.org/10.2174/0929866053405995 |
Print ISSN 0929-8665 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5305 |
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