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Protein & Peptide Letters


ISSN (Print): 0929-8665
ISSN (Online): 1875-5305

A General Procedure for the Production of Antibody Reagents Against Eukaryotic Ribosomal Proteins

Author(s): Giorgio Dieci, Lorena Bottarelli and Simone Ottonello

Volume 12, Issue 6, 2005

Page: [555 - 560] Pages: 6

DOI: 10.2174/0929866054395860

Price: $65


Despite recent progress in the structural and functional analysis of bacterial and archaeal ribosomes, the structure and biogenesis of eukaryotic ribosomes still awaits a detailed characterization. Ribosomal protein-specific antibodies would be valuable tools for such studies, but their production is commonly hindered by the poor expression and solubility of eukaryotic ribosomal proteins in E. coli. We report here an improved general procedure for the overproduction of recombinant eukaryotic ribosomal proteins and for the generation of the corresponding polyclonal antibodies. The specificity and sensitivity of detection of the antibodies produced by this procedure are documented.

Keywords: ribosomal protein, saccharomyces cerevisiae, overexpression, argu, escherichia coli, polyclonal antibodies

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