Discovery of Thrombin Inhibitor Fragments from Chemical Microarray Screening

Title: Discovery of Thrombin Inhibitor Fragments from Chemical Microarray Screening

Volume: 2 Issue: 8

Author(s): Thomas Neumann, Hans-Dieter Junker, Oliver Keil, Klaus Burkert, Holger Ottleben, Jurgen Gamer, Renate Sekul, Holger Deppe, Achim Feurer, Dirk Tomandl and Gunther Metz

Affiliation:

Keywords: Fragment discovery, chemical microarrays, surface plasmon resonance, thrombin, non-basic S1 binder

Abstract: Surface plasmon resonance imaging, a low affinity screening method, allows the highly parallel detection of small molecules binding to a target protein. The screening of a fragment based compound library immobilized on chemical microarrays resulted in the discovery of binding fragments for the serine protease thrombin. Functional assays confirmed enzymatic inhibition of microarray hits and crystallography established the binding mode of a non-basic S1 motif providing a starting point for medicinal chemistry.

Cite this article as:

Neumann Thomas, Junker Hans-Dieter, Keil Oliver, Burkert Klaus, Ottleben Holger, Gamer Jurgen, Sekul Renate, Deppe Holger, Feurer Achim, Tomandl Dirk and Metz Gunther, Discovery of Thrombin Inhibitor Fragments from Chemical Microarray Screening, Letters in Drug Design & Discovery 2005; 2 (8) . https://dx.doi.org/10.2174/157018005774717343