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Protein & Peptide Letters


ISSN (Print): 0929-8665
ISSN (Online): 1875-5305

Production of Recombinant Human Osteoprotegrin from Trichoplusia Ni Cells and Bombyx Mori Larvae

Author(s): Zhen Liu, Guan-zhen Yang and Xiang-Fu Wu

Volume 11, Issue 4, 2004

Page: [317 - 323] Pages: 7

DOI: 10.2174/0929866043406904

Price: $65


Human osteoprotegrin (OPG) and its truncated mutant OPG-280 and lengthened mutant OPG-Fc were constructed and successfully expressed in Trichoplusia ni cells and Bombyx mori larvae. Native SDSPAGE and Western blot analysis revealed that OPG-Fc is present as a homodimer in Tn cells or B. mori larvae compared with OPG and OPG-280. Furthermore, the hypocalcemic effect assay showed that truncation of the C-terminal 100 residues OPG does not abolish the biological activity and Fc can be helpful in forming the OPG homodimer with improved biological activity.

Keywords: opg, mutant, trichoplusia ni cell, bombyx mori larvae

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