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Current Protein & Peptide Science


ISSN (Print): 1389-2037
ISSN (Online): 1875-5550

TAT-Liposomes: A Novel Intracellular Drug Carrier

Author(s): V. P. Torchilin and T. S. Levchenko

Volume 4, Issue 2, 2003

Page: [133 - 140] Pages: 8

DOI: 10.2174/1389203033487298

Price: $65


TAT peptide was attached to the surface of plain and PEGylated liposomes. These TAT peptide-modified liposomes have been shown to translocate into a variety of normal and cancer cells if a non-hindered interaction between the cell surface and liposome-attached TAT peptide was made possible. TAT peptide-liposomes translocated into cells remain intact within first few hours as proved by a co-localization of fluorescent markers entrapped inside liposomes and incorporated into the liposomal membrane. After 2 hours liposomes had slowly migrating towards cell nuclei. Liposomes had completely disintegrated with their inner marker released by approximately 9 hours. TAT peptide-liposomes were made slightly cationic by adding up to 10 mol % of a cationic lipid (DOTAP). These slightly cationic liposomes were non-toxic towards cells, formed firm complexes with DNA (plasmid encoding for the formation of the Green Fluorescent Protein), and efficiently transfected a variety of cells. TAT peptideliposomes can be considered as promising carriers for the non-endocytotic intracellular delivery of drugs and DNA.

Keywords: TAT-Liposomes, peptide, PEGylated, liposomes, non-endocytotic

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