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Protein & Peptide Letters


ISSN (Print): 0929-8665
ISSN (Online): 1875-5305

Cloning and Expression of Human Beta-defensin-2 Gene in Escherichia Coli

Author(s): Xiangming Fang, Li Peng, Zhinan Xu, Jinmin Wu and Peilin Cen

Volume 9, Issue 1, 2002

Page: [31 - 37] Pages: 7

DOI: 10.2174/0929866023409011

Price: $65


Human beta-defensin-2 (hBD-2), a small cationic peptide, exhibits a broad range of antimicrobial activity. It has been found to play important roles in innate and adaptive immune responses against microbial invasion. For the purposes of this study, hBD-2 gene was cloned from the lesions of human condyloma acuminatum. An expression vector was constructed and transformed into E. coli. hBD-2 was expressed as a fusion protein in both the soluble and insoluble forms, which was further confirmed by western blotting analysis.

Keywords: Human beta-defensin-2 (hBD-2), proinflammatory agents, anti-Gram-negative, transcription-polymerase chain reaction (RT-PCR), QIAprep Spin Minprep Kit, Chelating column, Coomassie brilliant blue G-250

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