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Protein & Peptide Letters


ISSN (Print): 0929-8665
ISSN (Online): 1875-5305

Molecular Cloning of Grass Carp Growth Hormone Receptor and Its Functional Interaction with Silver Carp Growth Hormone

Author(s): Wei Hu, Xia Chen, Gusong Yu, Yan Chen, Yan Shen, Ying Zhang and Yi Gong

Volume 16, Issue 3, 2009

Page: [320 - 323] Pages: 4

DOI: 10.2174/092986609787601679

Price: $65


A novel grass carp growth hormone receptor (gcGHR) cDNA was cloned by RT-PCR method. CHO-K1 cells were transfected with eukaryotic expression vector pcDNA3.0 containing gcGHR entire coding region, resulting in a stable CHO cell line expressing gcGHR (CHO-gcGHR). Bioactivity assay revealed that silver carp growth hormone (scGH) bound gcGHR with dissociation constant (Kd) value of 1.96 nM and stimulated CHO-gcGHR cells proliferation with the sensitivity of 20 ng/ml. The cells proliferation assay was also provided to measure fish growth hormone levels of pituitary and refolded protein.

Keywords: cell proliferation, CHO-K1 cell, grass carp, growth hormone level, growth hormone receptor

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