Abstract
miRNAs are a new class of non-coding small RNAs that exist in many species. They play important roles in many physiological and pathophysiological processes by inhibiting the expression of target RNAs. Recent advances in miRNAs are beginning to be predicted and identified using several technological approaches, such as miRNA cloning, hybridization with various probes, and PCR-based detection. In the past few years, miRNA microarray technology has become the reference technique for monitoring the miRNA expression. In this review, miRNAs will be introduced and the characteristics of normalization methods in miRNA research will be discussed. The technical operations of miRNA profiling with microarrays will also be described, with an emphasis on probe design and labelling. The applications of the miRNA microarray in both basic and applied research will be summarized.
Keywords: miRNAs, profiling, technology, microarray
Current Bioinformatics
Title: miRNA Microarray Technology in miRNA Profiling
Volume: 4 Issue: 2
Author(s): Shu-Ting Wang, Cai Li and Lei Liu
Affiliation:
Keywords: miRNAs, profiling, technology, microarray
Abstract: miRNAs are a new class of non-coding small RNAs that exist in many species. They play important roles in many physiological and pathophysiological processes by inhibiting the expression of target RNAs. Recent advances in miRNAs are beginning to be predicted and identified using several technological approaches, such as miRNA cloning, hybridization with various probes, and PCR-based detection. In the past few years, miRNA microarray technology has become the reference technique for monitoring the miRNA expression. In this review, miRNAs will be introduced and the characteristics of normalization methods in miRNA research will be discussed. The technical operations of miRNA profiling with microarrays will also be described, with an emphasis on probe design and labelling. The applications of the miRNA microarray in both basic and applied research will be summarized.
Export Options
About this article
Cite this article as:
Wang Shu-Ting, Li Cai and Liu Lei, miRNA Microarray Technology in miRNA Profiling, Current Bioinformatics 2009; 4 (2) . https://dx.doi.org/10.2174/157489309788184783
DOI https://dx.doi.org/10.2174/157489309788184783 |
Print ISSN 1574-8936 |
Publisher Name Bentham Science Publisher |
Online ISSN 2212-392X |
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
Related Articles
-
A Review of Pharmacological Treatment Options for Lung Cancer: Emphasis on Novel Nanotherapeutics and Associated Toxicity
Current Drug Targets Stabilization of Eukaryotic Topoisomerase II-DNA Cleavage Complexes
Current Topics in Medicinal Chemistry EDITORIAL(Hot Topic Integrated Therapeutic Approaches in the Treatment of Human Cancer)
Anti-Cancer Agents in Medicinal Chemistry Oncogenomic Approaches in Exploring Gain of Function of Mutant p53
Current Genomics Single-Photon Emission Computed Tomography Tracers for Predicting and Monitoring Cancer Therapy
Current Pharmaceutical Biotechnology State of the Art and Perspectives in Food Allergy (Part I): Diagnosis
Current Pharmaceutical Design Natural Products from Cyanobacteria with Antimicrobial and Antitumor Activity
Current Pharmaceutical Biotechnology Intracellular Trafficking of MDR Transporters and Relevance of SNPs
Current Topics in Medicinal Chemistry Recent Progress in the Development of Synthetic Hybrids of Natural or Unnatural Bioactive Compounds for Medicinal Chemistry
Mini-Reviews in Medicinal Chemistry Epigenetic Modifications Due to Heavy Metals Exposure in Children Living in Polluted Areas
Current Genomics Imaging of HER-2 Overexpression in Tumors for Guiding Therapy
Current Pharmaceutical Design Nandrolone Decanoate Enhances the Activities of Cholanthrene Induced Glutathione-s-Transferase in Liver Tissue of Albino Mice
Drug Metabolism Letters Reactive Oxygen Species, Inflammation, and Lung Diseases
Current Pharmaceutical Design Cell Free DNA as an Evolving Liquid Biopsy Biomarker for Initial Diagnosis and Therapeutic Nursing in Cancer- An Evolving Aspect in Medical Biotechnology
Current Pharmaceutical Biotechnology Engineering of Plant Metabolism for Drug and Food
Current Medicinal Chemistry - Immunology, Endocrine & Metabolic Agents Core Functional Sequence of C-terminal GAG-binding Domain Directs Cellular Uptake of IGFBP-3-derived Peptides
Protein & Peptide Letters Preface
Current Pharmaceutical Design Oxytocin as a Potential Adjuvant Against COVID-19 Infection
Endocrine, Metabolic & Immune Disorders - Drug Targets The Research Progress of Taxol in <i>Taxus</i>
Current Pharmaceutical Biotechnology The Circulating Endothelial Cell in Cancer: Towards Marker and Target Identification
Current Pharmaceutical Design