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Current Proteomics


ISSN (Print): 1570-1646
ISSN (Online): 1875-6247

ELISA-based Protein Arrays: Multiplexed Sandwich Immunoassays

Author(s): Ruo-Pan Huang, Ying Lin, Li-Pai Chen, Weimin Yang and Ruochun Huang

Volume 1, Issue 3, 2004

Page: [199 - 210] Pages: 12

DOI: 10.2174/1570164043379226

Price: $65


Protein arrays with ability to simultaneously detect multiple protein expression levels, protein-protein interactions, protein-DNA or RNA interactions, protein-small molecule interactions and protein modifications will be an excellent tool in biomedical discovery. A variety of technologies have been developed to meet this need. In general, these assay systems can be divided into two major categories: label-based approaches, and sandwich or enzyme linked immunosorbent assay (ELISA)-based approaches. The purpose of this review is to provide an overview of the principles and techniques of ELISA-based protein arrays, with particular emphasis on the use of ELISA-based protein arrays for detection of multiple protein expression levels.

Keywords: protein arrays, antibody arrays, proteomics, elisa, protein expression, immunoassay, cytokine

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