Abstract
α-defensins are reported to be a soluble component of innate immunity actively participating in host defense against HIV. In order to further investigate the role of α-defensins in innate immunity during HIV infection, we analyzed CD8+ T lymphocytes and neutrophils obtained from 34 HIV-infected and 14 uninfected subjects. CD8+ T cells and neutrophils were labelled for evaluating α-defensin expression by flow cytometric analysis using a dual laser FACScalibur. Culture supernatants and plasma were also collected for ELISA quantification of α-defensins. The results showed a significantly increased production of α-defensins in plasma, neutrophils and CD8 T-lymphocytes of patients with HIV infection in comparison with healthy controls. The expression of α -defensins, by CD8+ cells probably reflects both the intrinsic production and the uptake from cocultured cells that release defensins. The upregulation of α-defensin expression within neutrophils could account for the increased release of such peptides in the systemic circulation. Antiretroviral treatment did not have any effect on plasma levels and expression of α-defensins by neutrophils. Overall, our findings suggest that the increased production/expression of α-defensins could be correlated with the chronic process of immune activation seen in HIV infection.
Keywords: HIV, α-defensins, innate immunity, CD8, neutrophils
Current HIV Research
Title: In Vivo Release of Alpha-Defensins in Plasma, Neutrophils and CD8 TLymphocytes of Patients with HIV Infection
Volume: 7 Issue: 6
Author(s): Claudia D'Agostino, Miriam Lichtner, Claudio M. Mastroianni, Giancarlo Ceccarelli, Marco Iannetta, Simone Antonucci, Vincenzo Vullo and Anna P. Massetti
Affiliation:
Keywords: HIV, α-defensins, innate immunity, CD8, neutrophils
Abstract: α-defensins are reported to be a soluble component of innate immunity actively participating in host defense against HIV. In order to further investigate the role of α-defensins in innate immunity during HIV infection, we analyzed CD8+ T lymphocytes and neutrophils obtained from 34 HIV-infected and 14 uninfected subjects. CD8+ T cells and neutrophils were labelled for evaluating α-defensin expression by flow cytometric analysis using a dual laser FACScalibur. Culture supernatants and plasma were also collected for ELISA quantification of α-defensins. The results showed a significantly increased production of α-defensins in plasma, neutrophils and CD8 T-lymphocytes of patients with HIV infection in comparison with healthy controls. The expression of α -defensins, by CD8+ cells probably reflects both the intrinsic production and the uptake from cocultured cells that release defensins. The upregulation of α-defensin expression within neutrophils could account for the increased release of such peptides in the systemic circulation. Antiretroviral treatment did not have any effect on plasma levels and expression of α-defensins by neutrophils. Overall, our findings suggest that the increased production/expression of α-defensins could be correlated with the chronic process of immune activation seen in HIV infection.
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D'Agostino Claudia, Lichtner Miriam, Mastroianni M. Claudio, Ceccarelli Giancarlo, Iannetta Marco, Antonucci Simone, Vullo Vincenzo and Massetti P. Anna, In Vivo Release of Alpha-Defensins in Plasma, Neutrophils and CD8 TLymphocytes of Patients with HIV Infection, Current HIV Research 2009; 7 (6) . https://dx.doi.org/10.2174/157016209789973600
DOI https://dx.doi.org/10.2174/157016209789973600 |
Print ISSN 1570-162X |
Publisher Name Bentham Science Publisher |
Online ISSN 1873-4251 |
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