Abstract
The peptide sequence KLVFFAE that spans the region 16-22 in the amyloid peptide Aβ1-40 has the ability to form fibrils or nanotubes in aqueous medium, depending on the conditions of dissolution. Interaction between the phenylalanine residues is presumed to play an important role in the self-assembly of Aβ16-22. We have investigated the importance of these aromatic residues by substituting them with p-chloro-, p-fluoro- and p-methylphenylalanine. Nanostructures different from the parent peptide were obtained with the substituted analogs, both in methanol as well as aqueous conditions (pH 2 and pH 7). Concentration-dependent effects observed in methanol, suggest that intermediate states occur during fibrillation. A balance between the crucial parameters such as charge, hydrophobicity and steric constraints implicated in self assembly, appear to modulate the nanostructure formation.
Keywords: Aβ16-22, aggregation, amyloid-like structure, derivatized-phenylalanines, nanostructures, self-assembly
Protein & Peptide Letters
Title:Effect of Replacing Phenylalanine Residues by Para-Substituted Phenylalanines on the Aggregation Behavior of Aβ16-22
Volume: 19 Issue: 5
Author(s): Chandrasekaran Sivakamasundari, Sasi Nandakumar and Ramakrishnan Nagaraj
Affiliation:
Keywords: Aβ16-22, aggregation, amyloid-like structure, derivatized-phenylalanines, nanostructures, self-assembly
Abstract: The peptide sequence KLVFFAE that spans the region 16-22 in the amyloid peptide Aβ1-40 has the ability to form fibrils or nanotubes in aqueous medium, depending on the conditions of dissolution. Interaction between the phenylalanine residues is presumed to play an important role in the self-assembly of Aβ16-22. We have investigated the importance of these aromatic residues by substituting them with p-chloro-, p-fluoro- and p-methylphenylalanine. Nanostructures different from the parent peptide were obtained with the substituted analogs, both in methanol as well as aqueous conditions (pH 2 and pH 7). Concentration-dependent effects observed in methanol, suggest that intermediate states occur during fibrillation. A balance between the crucial parameters such as charge, hydrophobicity and steric constraints implicated in self assembly, appear to modulate the nanostructure formation.
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Sivakamasundari Chandrasekaran, Nandakumar Sasi and Nagaraj Ramakrishnan, Effect of Replacing Phenylalanine Residues by Para-Substituted Phenylalanines on the Aggregation Behavior of Aβ16-22, Protein & Peptide Letters 2012; 19 (5) . https://dx.doi.org/10.2174/092986612800191044
DOI https://dx.doi.org/10.2174/092986612800191044 |
Print ISSN 0929-8665 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5305 |
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