Abstract
The secondary structure of a new type of recombinant RGD-hirudin, which has the activities of anti-thrombin and anti-platelet aggregation, has been studied by Fourier transform infrared spectroscopy (FT-IR), Raman spectroscopy and circular dichroism (CD) methods. The distribution of various secondary structure elements was determined using only a very small amount of sample protein. It was found that the recombinant RGD-hirudin contains about 26% extended chain, 21% β-turn and 53% unordered structure, leaving no α-helix. The results showed that the regular secondary structure of recombinant RGD-hirudin is increased compared with wild-type hirudin. The RGD segment that is located at the end of a long arm of a β-sheet is thought to play an important role in the additional function of anti-platelet aggregation. Throughout the experiments, FT-IR, Raman spectroscopy and CD generated mutually reinforcing results.
Keywords: Recombinant RGD-hirudin, FT-IR, Raman spectroscopy, CD, secondary structure
Protein & Peptide Letters
Title: Structure Study of Recombinant RGD-Hirudin by Vibrational and Circular Dichroism Spectroscopy
Volume: 13 Issue: 1
Author(s): X. Liu, W. Mo, L. Dai, X. Yan and H. Song
Affiliation:
Keywords: Recombinant RGD-hirudin, FT-IR, Raman spectroscopy, CD, secondary structure
Abstract: The secondary structure of a new type of recombinant RGD-hirudin, which has the activities of anti-thrombin and anti-platelet aggregation, has been studied by Fourier transform infrared spectroscopy (FT-IR), Raman spectroscopy and circular dichroism (CD) methods. The distribution of various secondary structure elements was determined using only a very small amount of sample protein. It was found that the recombinant RGD-hirudin contains about 26% extended chain, 21% β-turn and 53% unordered structure, leaving no α-helix. The results showed that the regular secondary structure of recombinant RGD-hirudin is increased compared with wild-type hirudin. The RGD segment that is located at the end of a long arm of a β-sheet is thought to play an important role in the additional function of anti-platelet aggregation. Throughout the experiments, FT-IR, Raman spectroscopy and CD generated mutually reinforcing results.
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Cite this article as:
Liu X., Mo W., Dai L., Yan X. and Song H., Structure Study of Recombinant RGD-Hirudin by Vibrational and Circular Dichroism Spectroscopy, Protein & Peptide Letters 2006; 13 (1) . https://dx.doi.org/10.2174/092986606774502135
DOI https://dx.doi.org/10.2174/092986606774502135 |
Print ISSN 0929-8665 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5305 |
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