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Current HIV Research

Editor-in-Chief

ISSN (Print): 1570-162X
ISSN (Online): 1873-4251

In Vitro Infection of Human Umbilical Cord Blood CD34+ Hematopoietic Progenitor Cells by HIV-1 CRF07_BC Enveloped Pseudovirus

Author(s): Lin Li, Chao Qiu, Liangzhu Li, Aiping Liu, Mingzhe Zhou, Zhimin Han, Chenli Qiu, Xiaoyan Zhang, Jianqing Xu and Huanzhang Zhu

Volume 10, Issue 7, 2012

Page: [572 - 577] Pages: 6

DOI: 10.2174/157016212803305989

Price: $65

Abstract

To determine whether CRF07_BC, one of the most predominant strains that accounts for one third HIV-1 prevalence in China, has the ability to infect hematopoietic progenitor cells (HPCs), human Umbilical Cord Blood (UCB) derived CD34+ HPCs isolated with high purity were infected by HIV-1 pseudotyped with CRF07_BC envelope. After HIV-1 infection, ~0.86% CD34+ HPCs were co-stained for CD34 and intracellular HIV Gag. HIV p24 antigen was detectable and reached maximal release between day 2-4 after HIV-1 infection. The data of nested Alu-LTR PCR proved the integration of HIV-1 genome into the host genome occurred in HIV-1-infected HPCs. These data demonstrated that the envelope of CRF07_BC from China has the capability of resulting in infection to CD34+ HPCs, which may serve as a mechanism for long-term latency of HIV-1 infection in vivo.

Keywords: HIV-1, latency, hematopoietic progenitor cells, CRF07_BC, integration


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