Generic placeholder image

Medicinal Chemistry


ISSN (Print): 1573-4064
ISSN (Online): 1875-6638

Antimicrobial Susceptibility of Aeromonas Spp. Isolated from Pig Ileum Segments to Natural Isothiocyanates

Author(s): Alfredo Aires, Carla S.P. Dias, Eduardo A. S. Rosa and Maria Jose Saavedra

Volume 9, Issue 6, 2013

Page: [861 - 866] Pages: 6

DOI: 10.2174/1573406411309060010

Price: $65


With this study we intend to evaluate the effects of 8 different glucosinolate hydrolysis products including isothiocyanates as antibacterial compounds against Aeromonadaceae, isolated from intestinal segments of pigs collected directly from slaughter-houses in the North of Portugal. Four Aeromonas species, A. allosaccharophila, A. hydrophila, A. media and A. veronii were identified. Using disc-diffusion bioassay all compounds were tested at 6 different doses (0.015, 0.15, 0.75, 1.5 and 3.0 µmoles) in dimethyl-sulfoxide (DMSO). Antibiotic standard (gentamicin) and DMSO (negative control) were included in all experiments. Minimum and maximum dose inhibitions (in µmoles) were assessed. To our knowledge, this is the first study of antimicrobial activity of glucosinolate hydrolysis products against Aeromonas species. The results showed that glucosinolate hydrolysis products and particularly the isothiocyanates have antimicrobial activity, which was proportional to the concentration used. However, not all revealed the same tendency, which means that the chemical structure of each compound is fundamental to understand their effectiveness. Among the different isothiocyanates the benzylisothiocyanate, sulforaphane and 2-phenylethylisothiocyanate were the most effective in vitro inhibitors of bacterial growth. This in vitro study provides enough data to demonstrate the potential use of these natural dietary chemicals for treating infectious diseases caused by Aeromonas spp.

Keywords: Aeromonadaceae, antibacterial activity, drug screening, isothiocyanates, natural products.

Rights & Permissions Print Export Cite as
© 2023 Bentham Science Publishers | Privacy Policy