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Current Analytical Chemistry

Editor-in-Chief

ISSN (Print): 1573-4110
ISSN (Online): 1875-6727

Ligand Screening by Saturation-Transfer Difference (STD) NMR Spectroscopy

Author(s): V. V. Krishnan

Volume 1, Issue 3, 2005

Page: [307 - 320] Pages: 14

DOI: 10.2174/157341105774573956

Price: $65

Abstract

NMR based methods to screen for high-affinity ligands have become an indispensable tool for designing rationalized drugs, as these offer a combination of good experimental design of the screening process and data interpretation methods, which together provide unprecedented information on the complex nature of protein-ligand interactions. These methods rely on measuring direct changes in the spectral parameters, that are often simpler than the complex experimental procedures used to study structure and dynamics of proteins. The goal of this review article is to provide the basic details of NMR based ligand-screening methods, with particular focus on the saturation transfer difference (STD) experiment. In addition, we provide an overview of other NMR experimental methods and a practical guide on how to go about designing and implementing them.

Keywords: nuclear magnetic resonance (nmr), ligand screening, saturation transfer difference (std), protein, nuclear overhauser effect (noe) and relaxation


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