Saccharide microarray technology has emerged as a powerful tool for studying the interactions of saccharides with a variety of biomolecules in high-throughput manner. An efficient and selective immobilization technique of saccharides on the surface is essential for successful preparation of saccharide microarrays. Carbohydrates can be immobilized on the support surface by covalent or noncovalent binding. The methods of preparing saccharide microarrays were summarized herein.
Keywords: Covalent or noncovalent immobilization, preparation, saccharide microarrays, saccharide-protein interactions.