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Protein & Peptide Letters


ISSN (Print): 0929-8665
ISSN (Online): 1875-5305

Applications of “Tender” Energy (1-5 keV) X-ray Absorption Spectroscopy in Life Sciences

Author(s): Paul Northrup, Alessandra Leri and Ryan Tappero

Volume 23, Issue 3, 2016

Page: [300 - 308] Pages: 9

DOI: 10.2174/0929866523666160107114505

Price: $65


The “tender” energy range of 1 to 5 keV, between the energy ranges of most “hard” (>5 keV) and “soft” (<1 keV) synchrotron X-ray facilities, offers some unique opportunities for synchrotron- based X-ray absorption fine structure spectroscopy in life sciences. In particular the K absorption edges of Na through Ca offer opportunities to study local structure, speciation, and chemistry of many important biological compounds, structures and processes. This is an area of largely untapped science, in part due to a scarcity of optimized facilities. Such measurements also entail unique experimental challenges. This brief review describes the technique, its experimental challenges, recent progress in development of microbeam measurement capabilities, and several highlights illustrating applications in life sciences.

Keywords: Calcium, EXAFS, microbeam, natural organochlorine, sulfur, tender-energy, XANES, X-ray absorption spectroscopy, X-ray spectromicroscopy.

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