摘要
目的:探讨超顺磁性氧化铁(SPIO)纳米粒子(SPIO)作为磁共振(MR)造影剂增强体内肿瘤显像的可行性。 方法:疏水SPIO(油溶性超顺磁性氧化铁,ospio)和亲水性的超顺磁性氧化铁(SPIO水溶液。wspio)装载在甲氧基聚(乙二醇)-b-聚(D,L-乳酸)(PEG-PDLLA)纳米囊泡。三组被移植人结肠癌(LoVo)细胞的裸鼠(n = 12 /组)被注入wspio、ospio纳米载药囊泡或wspio-纳米载药囊泡至尾静脉。MRI扫描所有的小鼠,在肿瘤和肝中测量相关T2值,比较三组患者在T2值上的差异。 结果:峰相对T值由ospio-或wspio-装载造影剂检测肿瘤分别减少了10.12%和11.40%。相对T2值在wspio和ospio加载聚合物纳米载药囊泡组比在wspio组更加明显(P<0.05),但T2值在ospio和wspio装囊组之间的差异无统计学意义(P>0.05)。在肝脏中最大的T2值减小值在wspio组、ospio和wspio装囊组分别为32.85%、52.77%和56.89%。T2值的下降在wspio装载组和ospio纳米载药囊泡组比在wspio组明显(P<0.05),其中wspio纳米载药囊泡组比在ospio纳米载药囊泡组更明显(P<0.05)。 结论:SPIO聚合物纳米囊泡内产生显著的T2WI信号强度降低,预计将作为新的有效的肿瘤成像造影剂。
关键词: 超顺磁性氧化铁;自组装;聚合;nanovesicle;磁共振成像;大肠癌
Current Cancer Drug Targets
Title:Molecular Targeted Magnetic Resonance Imaging of Human Colorectal Carcinoma (LoVo) Cells Using Novel Superparamagnetic Iron Oxide- Loaded Nanovesicles: In Vitro and in vivo Studies
Volume: 16 Issue: 6
Author(s): Shi-Ting Feng, Hao Li, Yanji Luo, Huasong Cai, Zhi Dong, Zhuangnian Fang, Xintao Shuai, Zi-Ping Li
Affiliation:
关键词: 超顺磁性氧化铁;自组装;聚合;nanovesicle;磁共振成像;大肠癌
摘要: Objective: To investigate the feasibility of superparamagnetic iron oxide (SPIO) nanoparticles (SPIO) as a magnetic resonance (MR) contrast agent to enhance tumor imaging in vivo.
Methods: Hydrophobic SPIO (oil-soluble SPIO; OSPIO) and hydrophilic SPIO (water-soluble SPIO; WSPIO) were loaded in methoxy-poly(ethylene glycol)-block-poly(D,L-lactic acid) (PEG-PDLLA) nanovesicles. Three groups of nude mice (n=12/group) xenografted with human colorectal carcinoma (LoVo) cells were injected into the caudal vein with WSPIO, OSPIO-loaded nanovesicles, or WSPIO-loaded nanovesicles. MRI scans were performed on all of the mice, and the relative T2 values were measured in the tumor and the liver. The differences in these T2 values between the three groups were compared.
Results: The peak relative T2 values in the tumors detected by the OSPIO- or WSPIO-loaded contrast agents were reduced by 10.12% and 11.40%, respectively. The relative T2 values in the WSPIO- and OSPIO-loaded polymeric nanovesicle groups were more pronounced than the relative T2 value in the WSPIO group (P<0.05), but there was no significant difference in the T2 value between the OSPIO- and WSPIO-loaded vesicle groups (P>0.05). The greatest T2 value decreases in the liver in the WSPIO, OSPIO-loaded and WSPIO-loaded vesicle groups were 32.85%, 52.77% and 56.89%, respectively. The decrease in the T2 values was more pronounced in the WSPIO- and OSPIO-loaded nanovesicle groups than in the WSPIO group (P<0.05) and was more apparent in the WSPIO-loaded nanovesicle group than in the OSPIO-loaded nanovesicle group (P<0.05).
Conclusion: SPIO-loaded polymeric nanovesicles generate significant T2WI signal intensity decreases in vivo and are anticipated to be used as novel and effective contrast agents for tumor imaging.
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Cite this article as:
Shi-Ting Feng, Hao Li, Yanji Luo, Huasong Cai, Zhi Dong, Zhuangnian Fang, Xintao Shuai, Zi-Ping Li , Molecular Targeted Magnetic Resonance Imaging of Human Colorectal Carcinoma (LoVo) Cells Using Novel Superparamagnetic Iron Oxide- Loaded Nanovesicles: In Vitro and in vivo Studies, Current Cancer Drug Targets 2016; 16 (6) . https://dx.doi.org/10.2174/1568009616666160603123616
DOI https://dx.doi.org/10.2174/1568009616666160603123616 |
Print ISSN 1568-0096 |
Publisher Name Bentham Science Publisher |
Online ISSN 1873-5576 |
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