Single-Molecule Spectroscopy for Nucleic Acid Analysis: A New Approach for Disease Detection and Genomic Analysis

Title: Single-Molecule Spectroscopy for Nucleic Acid Analysis: A New Approach for Disease Detection and Genomic Analysis

Volume: 5 Issue: 3

Author(s): Peter M. Goodwin, Rhiannon L. Nolan and Hong Cai

Affiliation:

Keywords: single-molecule, fluorescence, two-color, haplotyping, gene expression

Abstract: Recently developed single-molecule spectroscopy (SMS) permits the analysis of fluorescent mixtures one molecule at a time. SMS methods provide the means to make rapid measurements on small, complex samples without the need for separations and target amplification enabling a new class of ultrasensitive nucleic acid assays. Here we give a brief overview of the current state of the art of SMS nucleic acid analysis and discuss ongoing work in our laboratory on two-color single-molecule fluorescence detection of specific nucleic acid sequences. In the future, two-color SMS nucleic acid assays will be used for a variety of applications including: gene expression analysis, disease detection and genomics.

Cite this article as:

Goodwin M. Peter, Nolan L. Rhiannon and Cai Hong, Single-Molecule Spectroscopy for Nucleic Acid Analysis: A New Approach for Disease Detection and Genomic Analysis, Current Pharmaceutical Biotechnology 2004; 5 (3) . https://dx.doi.org/10.2174/1389201043376814