Abstract
The interactions of nucleic acids technology and the technology of arrayed nucleic acids are described, showing the interdependence of nucleic acids chemistry, surface chemistry, (micro-) technology and the requirements of biomedical applications. The methods and problems of the production of large numbers of oligonucleotides as well as the methods of arraying oligonucleotides are highlighted. The basic approaches, in-situ synthesis and postsynthetic immobilization, are described with a special emphasis on the postsynthetic immobilization of ready-made oligonucleotides on support materials. Techniques for the detection of nucleic acids interactions on arrays are outlined in brief.
Keywords: nucleic acids technology, oligonucleotide array, oligonucleotide chip, in-situ synthesis, immobilization, spotting, surface activation
Current Pharmaceutical Biotechnology
Title: Arrays of Immobilized Oligonucleotides - Contributions to Nucleic Acids Technology
Volume: 4 Issue: 6
Author(s): H. Seliger, M. Hinz and E. Happ
Affiliation:
Keywords: nucleic acids technology, oligonucleotide array, oligonucleotide chip, in-situ synthesis, immobilization, spotting, surface activation
Abstract: The interactions of nucleic acids technology and the technology of arrayed nucleic acids are described, showing the interdependence of nucleic acids chemistry, surface chemistry, (micro-) technology and the requirements of biomedical applications. The methods and problems of the production of large numbers of oligonucleotides as well as the methods of arraying oligonucleotides are highlighted. The basic approaches, in-situ synthesis and postsynthetic immobilization, are described with a special emphasis on the postsynthetic immobilization of ready-made oligonucleotides on support materials. Techniques for the detection of nucleic acids interactions on arrays are outlined in brief.
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Cite this article as:
Seliger H., Hinz M. and Happ E., Arrays of Immobilized Oligonucleotides - Contributions to Nucleic Acids Technology, Current Pharmaceutical Biotechnology 2003; 4 (6) . https://dx.doi.org/10.2174/1389201033377454
DOI https://dx.doi.org/10.2174/1389201033377454 |
Print ISSN 1389-2010 |
Publisher Name Bentham Science Publisher |
Online ISSN 1873-4316 |
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