Abstract
Fluorescence polarization immunoassay methods for the detection of pesticides and their metabolites or degradation products are reviewed. Advantages and limitations for application to pesticide detection in environmental and food samples are discussed. The influence of the structure of fluorescentlabeled tracers and the affinity and specificity of antibodies on analytical performance is examined. The methods are simple, readily automated, and rapid (total time for assay of a water sample is about 1 min) with sensitivity of 1 - 10 ng / ml pesticide in 0.01 - 0.1 ml sample.
Keywords: Fluorescence polarization immunoassay,, pesticide,, review.
Combinatorial Chemistry & High Throughput Screening
Title: Fluorescence Polarization Immunoassays for Pesticides
Volume: 6 Issue: 3
Author(s): Sergei A. Eremin and David S. Smith
Affiliation:
Keywords: Fluorescence polarization immunoassay,, pesticide,, review.
Abstract: Fluorescence polarization immunoassay methods for the detection of pesticides and their metabolites or degradation products are reviewed. Advantages and limitations for application to pesticide detection in environmental and food samples are discussed. The influence of the structure of fluorescentlabeled tracers and the affinity and specificity of antibodies on analytical performance is examined. The methods are simple, readily automated, and rapid (total time for assay of a water sample is about 1 min) with sensitivity of 1 - 10 ng / ml pesticide in 0.01 - 0.1 ml sample.
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Cite this article as:
Eremin A. Sergei and Smith S. David, Fluorescence Polarization Immunoassays for Pesticides, Combinatorial Chemistry & High Throughput Screening 2003; 6 (3) . https://dx.doi.org/10.2174/138620703106298301
DOI https://dx.doi.org/10.2174/138620703106298301 |
Print ISSN 1386-2073 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5402 |
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