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Current Medicinal Chemistry

Editor-in-Chief

ISSN (Print): 0929-8673
ISSN (Online): 1875-533X

Noninvasive Genotyping of Dopamine Receptor D4 (DRD4) UsingNanograms of DNA From Substance-Dependent Patients

Author(s): K. Boor, Z. Ronai, Z. Nemoda, P. Gaszner, M. Sasvari-Szekely, A. Guttman and H. Kalasz

Volume 9, Issue 8, 2002

Page: [793 - 797] Pages: 5

DOI: 10.2174/0929867024606821

Price: $65

Abstract

A noninvasive DNA sampling method has been implemented collecting buccalmucosa cells by cotton wool swabs. An amount of 0.2-2 μg DNA per patient wasobtained after the phenol-extraction procedure and 0.2-2 ng DNA template wassufficient for PCR amplification of the polymorphic 48 basepair repeat region of dopaminereceptor D4 (DRD4) gene. PCR products were visualized during microfabricated electrophoretic separationby laser-induced fluorescent detection and automatic data registration. Initial data of genotyping drugdependentsubjects shows a relatively high ratio of heterozygotes, possessing either longer or shorter variantsbeside the common 4-repeat DRD4 allele.

Keywords: doamine d4 recetor, genotyping, noninvasive dna samling, buccal swab

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