Generic placeholder image

Current Pharmacogenomics and Personalized Medicine


ISSN (Print): 1875-6921
ISSN (Online): 1875-6913

Research Article

Peculiaritie of Distribution of Polymorphic Variants of IL1Β Gene in Patients with Atherosclerosis and Metabolic Syndrome

Author(s): Saranchina Y. Vladimirovna*, Rossova N. Aleksandrovna, Khanarin N. Vladimirovich, Kilina O. Yur'evna, Dutova S. Vyacheslavovna and Kulakova T. Sergeevna

Volume 17, Issue 1, 2020

Page: [32 - 39] Pages: 8

DOI: 10.2174/1875692117666190416150346


Objective: The purpose of the study was to analyze the association of allelic polymorphism of IL1В gene C>T loci -31 and +3953 with atherosclerotic changes of artries in patients with Metabolic Syndrome (MS).

Materials and Methods: The main group of the study included 30 consecutive patients (24 women and 6 men, mean age - 51.7±2.2 years), for examination and treatment in the therapeutic Department of the Republican clinical hospital named "G. YA. Remishevskaya" (Abakan) about arterial hypertension or suspicion of type 2 diabetes. The criteria for inclusion in the core group included: compliance with the MS criteria according to the IDF criteria (2006); and the presence of ultrasound markers of Atherosclerosis (AS) according to the study of brachiocephalic arteries (presence of Atherosclerotic Plaques (ASP) and stenosis ≥30%). The control group included persons who underwent a planned medical examination in the Republican clinical hospital name "G. YA. Remishevskaya" (Abakan). A total of 35 patients (26 women and 9 men, mean age 44.7±1.5 years) were selected. The study involved the Russian population (Caucasians) living in the territory of the Republic of Khakassia. All the necessary examination and data collection were conducted including anamnestic data, anthropometric examination (measurements of length and body mass, waist circumference) body mass index, laboratory examination of blood biochemical parameters (glucose and lipid) and instrumental examination (blood pressure measurement, conducting ECG and ultrasound the brachiocephalic arteries). Single-nucleotide polymorphisms (SNP) of the promoter region of the IL1B gene at position-31C/T (rs1143627) and polymorphism in the coding part of the gene in exon 5 +3953C/T (rs 1143634) were studied by restriction analysis of amplification products (RFLP analysis).

Results: The risk of development of AS in patients with MS may be higher in carriers of genotype TT (OR = 1,76; 95% CI: (0,96-3,24)) or T allele (OR = 1,44; 95% CI: (0,82- 2,53)) IL1В gene in the polymorphic locus of the T-31С and genotype CT (OR = 1,85; 95% CI: (0,92-3,37)) or T allele (OR = 1,35; 95% CI: (0,63-2,89)) IL1В gene in the polymorphic locus of C + 3953T. The most common combination of gene polymorphisms IL1В was haplotype (-31) ТC/(+3953)СС in both the groups surveyed (40.6% to 36.8%, respectively). Variant (-31)TT/(+3953)CT in the main group was found significantly more often (15.8%, at χ2= 4.92, at p=0.03) than in the control group (3.1 %). The value of the odds ratio in this case was 3.99 (95% CI: (1.08-14.79), which indicates the risk of AS development against the background of MS in carriers of combined genotype inheritance (-31)TT/(+3953) CT.

Conclusion: The risk of development of AS in the background of MS is increased in carriers of combinations of SNPs (-31)TT/(+3953)CT IL1В gene responsible for hyperproduction of this cytokine. In this connection, further studies of the association of genes with MS and AS components should focus on intergenic interactions.

Keywords: Gene polymorphism, atherosclerosis, metabolic syndrome, interleukin-1B, IL1Β Gene, allelic polymorphism.

Graphical Abstract
Baptista D, Mach F, Brandt KJ. Follicular regulatory T cell in atherosclerosis. J Leukoc Biol 2018; 104(5): 925-30.
[] [PMID: 30134501]
Liu W, Chang C, Hu H, Yang H. Interleukin-23: A new atherosclerosis target. J Interferon Cytokine Res 2018; 38(10): 440-4.
[] [PMID: 30328797]
Nejati P, Naeimipour S, Salehi A, et al. Association of tumor necrosis factor-alpha gene promoter polymorphism and its mRNA expression level in coronary artery disease. Meta Gene 2018; 18: 122-6.
Sao R, Aronow WS. Association of non-alcoholic fatty liver disease with cardiovascular disease and subclinical atherosclerosis. Arch Med Sci 2018; 14(6): 1233-44.
[] [PMID: 30393477]
Riahi SM, Moamer S, Namdari M, Mokhayeri Y, Pourhoseingholi MA, Hashemi-Nazari SS. Patterns of clustering of the metabolic syndrome components and its association with coronary heart disease in the Multi-Ethnic Study of Atherosclerosis (MESA): A latent class analysis. Int J Cardiol 2018; 271: 13-8.
[] [PMID: 29885819]
Chai JT, Ruparelia N, Goel A, et al. Differential gene expression in macrophages from human atherosclerotic plaques shows convergence on pathways implicated by genome-wide association study risk variants. Arterioscler Thromb Vasc Biol 2018; 38(11): 2718-30.
[] [PMID: 30354237]
Kim M, Kim M, Yoo HJ, Bang YJ, Lee SH, Lee JH. Apolipoprotein A5 gene variants are associated with decreased adiponectin levels and increased arterial stiffness in subjects with low high-density lipoprotein-cholesterol levels. Clin Genet 2018; 94(5): 438-44.
[] [PMID: 30132804]
Khan R, Rheaume E, Tardif JC. Examining the role of and treatment directed at IL-1 in atherosclerosis. Curr Atheroscler Rep 2018; 20(11): 53.
[] [PMID: 30219977]
Fang Y, Xie H, Lin Z. Association between IL-1β + 3954C/T polymorphism and myocardial infarction risk: A meta-analysis. Medicine (Baltimore) 2018; 97(30) e11645
[] [PMID: 30045312]
George S, Zimmet P, Shaw J, Grundy SM. The IDF consensus worldwide definition of the metabolic syndrome. Belgium: International Diabetes Federation 2006.
Touboul PJ, Hennerici MG, Meairs S, et al. Mannheim carotid intima-media thickness and plaque consensus (2004-2006-2011). An update on behalf of the advisory board of the 3rd, 4th and 5th watching the risk symposia, at the 13th, 15th and 20th European Stroke Conferences, Mannheim, Germany, 2004, Brussels, Belgium, 2006, and Hamburg, Germany, 2011. Cerebrovasc Dis 2012; 34(4): 290-6.
[] [PMID: 23128470]
Khan NS, Allai MS, Nissar B, et al. Genetic association of Tumour necrosis factor alpha, Interleukin-18 and Interleukin 1 beta with the risk of coronary artery disease: A case-control study outcome from Kashmir. J Appl Biomed 2018; 16(4): 387-93.
Jager J, Grémeaux T, Cormont M, Le Marchand-Brustel Y, Tanti JF. Interleukin-1beta-induced insulin resistance in adipocytes through down-regulation of insulin receptor substrate-1 expression. Endocrinology 2007; 148(1): 241-51.
[] [PMID: 17038556]
Manica-Cattani MF, Duarte MM, Ribeiro EE, de Oliveira R, Mânica da Cruz IB. Effect of the interleukin-1B gene on serum oxidized low-density lipoprotein levels. Clin Biochem 2012; 45(9): 641-5.
[] [PMID: 22425603]
Maintinguer Norde M, Oki E, Ferreira Carioca AA, et al. Influence of IL1B, IL6 and IL10 gene variants and plasma fatty acid interaction on metabolic syndrome risk in a cross-sectional population-based study. Clin Nutr 2018; 37(2): 659-66.
[] [PMID: 28268030]
Suzuki K, Inoue T, Yanagisawa A, Kimura A, Ito Y, Hamajima N. Association between Interleukin-1B C-31T polymorphism and obesity in Japanese. J Epidemiol 2009; 19(3): 131-5.
[] [PMID: 19398847]
Rechciński T, Grebowska A, Kurpesa M, et al. Interleukin-1b and interleukin-1 receptor inhibitor gene cluster polymorphisms in patients with coronary artery disease after percutaneous angioplasty or coronary artery bypass grafting. Kardiol Pol 2009; 67(6): 601-10.
[PMID: 19618316]
Yanagisawa A, Suzuki K, Kimura A, Ito Y, Hamajima N, Inoue T. Possible protective effect of serum beta-carotene levels on the association between interleukin-1B C-31T polymorphism and hypertension in a Japanese population. Clin Nutr 2009; 28(2): 198-202.
[] [PMID: 19249142]
Soylu O, Yildirim A, Coker A, Tezel T, List EO, Arman A. Interleukin-1B (-511) gene polymorphism is associated with acute coronary syndrome in the Turkish population. Eur Cytokine Netw 2008; 19(1): 42-8.
[PMID: 18299273]
Yang HT, Wang SL, Yan LJ, et al. Association of interleukin gene polymorphisms with the risk of coronary artery disease. Genet Mol Res 2015; 16; 14(4): 12489-96.

© 2024 Bentham Science Publishers | Privacy Policy