Chemical Fingerprints of Medicinal Plants - HPTLC Profiling

Process Steps for HPTLC Analysis

Author(s): Acharya Balkrishna * .

Pp: 12-16 (5)

DOI: 10.2174/9789815196412123010004

* (Excluding Mailing and Handling)


This chapter briefly discusses the general aspects that can affect the result of TLC. Sample preparation, application of samples, preparation of development solvents, the steps involved in the development of TLC, visualization and derivatization, and detection of analyte are the key steps of chromatographic development. This chapter focuses on the process steps for HPTLC analysis.

Keywords: Derivatization, Detection, Stationary phase, Visualization.

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