Next-Generation Sequencing and Sequence Data Analysis

Paired-End (PE), Mate Pair (MP) and Paired-End Ditag (PED) Sequencing

Author(s): Kuo Ping Chiu

Pp: 62-76 (15)

DOI: 10.2174/9781681080925115010011

* (Excluding Mailing and Handling)


Information regarding the distance between paired reads enhances the accuracy of genome assembly and sequence-to-genome mapping, making paired-end indispensable strategies for DNA sequencing. The most commonly used paired-end sequencing strategies are Paired-End (PE) sequencing and Paired-End Ditag (PED) sequencing. Similarity in terminologies frequently causes confusion. This chapter is set out to clarify these terminologies and then, using PED as an example, to illustrate how a biotechnology can be sequentially developed.

Keywords: bPED, ChIP-EM, ChIP-HM, ChIP-TFBS, in situ PCR, mbPED, PE sequencing, PED sequencing.

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