Next-Generation Sequencing and Sequence Data Analysis

Single Cell Sequencing (SCS) and Single Cell Transcriptome (SCT) Sequencing

Author(s): Kuo Ping Chiu

Pp: 97-104 (8)

DOI: 10.2174/9781681080925115010015

* (Excluding Mailing and Handling)


Sometimes genomic and transcriptomic information of single cells, instead of those produced from cell populations, are desired. Obtaining such information relies on single cell sequencing (SCS) and single cell transcriptome (SCT) sequencing.

Although SCT sequencing is in fact part of SCS, they are readily distinguishable not only in research objective, but also in experimental procedure and bioinformatic approach. We will first review the history and achievements that have been made in these fields, and then discuss an experimental procedure of SCT sequencing to gain more insight into the subject.

Keywords: SCS, Single cell Sequencing, Single cell transcriptome sequencing, SCT, Transcriptome.

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