Abstract
The protein population secreted from three wine-contaminant microorganisms was studied by two-dimensional electrophoresis and screened for proteases. Proteolytic enzymes were electrophoretically purified and their activity, optimum pH and temperature determined. The protease of Acetobacter aceti maintained its activity over the range of pHs 3.0- 5.0, thus being of potential biotechnological interest.
Keywords: Lactobacillus hilgardii, Acetobacter aceti, Brettanomyces bruxellensis, extracellular protease, two-dimensional electrophoresis, zymogram
Protein & Peptide Letters
Title: Acidic Extracellular Proteases from Microrganisms of Fairly Acidic Niche
Volume: 13 Issue: 7
Author(s): Alessandra Bossi, Luca Bonizzato and Giacomo Zapparoli
Affiliation:
Keywords: Lactobacillus hilgardii, Acetobacter aceti, Brettanomyces bruxellensis, extracellular protease, two-dimensional electrophoresis, zymogram
Abstract: The protein population secreted from three wine-contaminant microorganisms was studied by two-dimensional electrophoresis and screened for proteases. Proteolytic enzymes were electrophoretically purified and their activity, optimum pH and temperature determined. The protease of Acetobacter aceti maintained its activity over the range of pHs 3.0- 5.0, thus being of potential biotechnological interest.
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Cite this article as:
Bossi Alessandra, Bonizzato Luca and Zapparoli Giacomo, Acidic Extracellular Proteases from Microrganisms of Fairly Acidic Niche, Protein & Peptide Letters 2006; 13 (7) . https://dx.doi.org/10.2174/092986606777790647
DOI https://dx.doi.org/10.2174/092986606777790647 |
Print ISSN 0929-8665 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5305 |
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