Abstract
The development of acute lung injury (ALI) is always accompanied by remarkably increased production of cytokines in plasma. However, the pathogenesis of this inflammatory response is unclear. In this study, the hypothesis whether Interleukin 33 (IL-33) is induced in the ALI patients and the possible mechanism was tested. The levels of IL-33 were found to be significantly induced in plasma of the ALI patients. Addition of IL-33, enhanced mRNA expression of NLRP-3, Caspase-3, BAX and p53 of peripheral mononuclear blood cells (PBMCs), but inhibited mRNA expression of Caspase-1 found in ALI patients. By stimulating the PBMCs with recombinant Human Interleukin (rhIL)-33, we demonstrated that the chemokines Interleukin 1β (IL-1β), Interleukin 6 (IL-6), Interleukin 18 (IL-18) and Tumor Necrosis Factor (TNF-α) were significantly increased with IL-33 administration in a dose dependent way. Concomitantly, the increase of phosphorylated p38 and enhancement of cell apoptosis were also found in the PBMCs. However, by blocking the IL-33 signaling with p38 inhibitor (SB 203580), the cytokine production and apoptosis were apparently blocked in PBMCs. The present study provided evidences for the IL-33 molecular mechanism in ALI patients. It promots cell apoptosis and cytokines production via the MAPK/p38 signaling in PBMCs.
Keywords: Acute lung injury (ALI), inflammation, IL-33, PBMCs, p38.
Call for Papers in Thematic Issues
Metabolism in Inflammation-Induced Cancer
We will talk about the primary mechanisms and principles of inflammation-induced cancer based on the most recent research findings and our understanding of the tumor development process, with an emphasis on the crosstalk between metabolism and inflammation-induced cancer. The regulatory mechanisms of T cell subpopulation differentiation, the regulatory function of ...read more
Related Journals
Related Books
Wild Vegetables: Morphology, Phytochemistry and Utility Part 1
Biology for Students
Medicinal Plants of Pilibhit Tiger Reserve (PTR) India
Plant-Microbe Interactions: A Comprehensive Review
Glycosylation and Glycation in Health and Diseases
Phytogenic and Phytochemical as Alternative Feed Additives for Animal Production
Methods and Techniques in Nematology
Mind Maps in Clinical Biochemistry
A Treatise on Ecological Science
From SARS-CoV to MARS-CoV
33
1
