Abstract
This review introduces the concept of enzyme biosensors. As the major feature of an enzyme is its selectivity and specificity, we briefly highlighted the principles of enzyme production and enzyme classification. Moreover, enzyme immobilization methods, including physical adsorption and entrapment techniques with special attention on cross-linking and covalent binding are discussed followed by different electrochemical methods used in the biosensors fabrication. Furthermore, a comprehensive discussion about horseradish peroxidase (HRP) as a model enzyme is presented with investigation of some modified enzyme electrodes such as screen-printed and carbon paste electrodes. Some approaches to enhance biosensors performances and their applications are also highlighted.
Keywords: Biosensors, enzyme, electrochemistry, immobilization techniques, modified electrodes.
Current Analytical Chemistry
Title:Recent Advances on Electrochemical Enzyme Biosensors
Volume: 12 Issue: 1
Author(s): Ezzaldeen Younes Jomma and Shou-Nian Ding
Affiliation:
Keywords: Biosensors, enzyme, electrochemistry, immobilization techniques, modified electrodes.
Abstract: This review introduces the concept of enzyme biosensors. As the major feature of an enzyme is its selectivity and specificity, we briefly highlighted the principles of enzyme production and enzyme classification. Moreover, enzyme immobilization methods, including physical adsorption and entrapment techniques with special attention on cross-linking and covalent binding are discussed followed by different electrochemical methods used in the biosensors fabrication. Furthermore, a comprehensive discussion about horseradish peroxidase (HRP) as a model enzyme is presented with investigation of some modified enzyme electrodes such as screen-printed and carbon paste electrodes. Some approaches to enhance biosensors performances and their applications are also highlighted.
Export Options
About this article
Cite this article as:
Jomma Younes Ezzaldeen and Ding Shou-Nian, Recent Advances on Electrochemical Enzyme Biosensors, Current Analytical Chemistry 2016; 12 (1) . https://dx.doi.org/10.2174/1573411011666150611184215
DOI https://dx.doi.org/10.2174/1573411011666150611184215 |
Print ISSN 1573-4110 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-6727 |
Call for Papers in Thematic Issues
Advanced Nanomaterials and Nanocomposites: Controlled Synthesis and Applications for Bioanalysis
In recent years, a range of novel nanomaterials have been developed and found broad applications in various fields. By controlling the synthesis process and modifying the nanomaterials, novel properties and functions can be tailored to form nanocomposites. This thematic issue aims to highlight the latest advances in the preparation of ...read more
Advancements in Biomedicine, Corrosion Inhibition/Monitoring, and Lubrication of Carbon Dots
Carbon dots, a class of carbon-based nanomaterials, have garnered substantial interest in various scientific and engineering fields due to their unique properties, including excellent optical properties, outstanding biocompatibility, easy preparation, good dispersity, ultrasmall size, low toxicity and environmental friendliness. This special issue of Current Analytical Chemistry aims to showcase cutting-edge ...read more
Advances in Microfluidic and Sensing Technologies of Biofluids
Detection of biological fluids is an important means to assess human health and diagnose diseases. Currently, research on blood is relatively mature and widely applied in the medical field. With the development and advancement of microfluidic technology and novel detection methods, attention has turned to human physiological fluids such as ...read more
Analytical Methods for Environmental and Food Analysis
In recent years, the study of the chemical composition of food and the environment has depended on analytical methods in order to acquire information about the chemical composition, processing, quality control, and contamination of soil, water, air, plants, and foods. These aspects are very important for food safety and environmental ...read more

- Author Guidelines
- Bentham Author Support Services (BASS)
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
Related Articles
-
Preface [ Infectious Disorders Drug Targets - An Arsenal of Knowledge on Pathogen Targets ]
Infectious Disorders - Drug Targets Myeloperoxidase as a Target for the Treatment of Inflammatory Syndromes: Mechanisms and Structure Activity Relationships of Inhibitors
Current Medicinal Chemistry Deciphering the Neuroprotective Role of Glucagon-like Peptide-1 Agonists in Diabetic Neuropathy: Current Perspective and Future Directions
Current Protein & Peptide Science Biological Activities of Receptor-interacting Protein 140 in Adipocytes and Metabolic Diseases
Current Diabetes Reviews Telmisartan Inhibits Advanced Glycation End Products (AGEs)-Elicited Endothelial Cell Injury by Suppressing AGE Receptor (RAGE) Expression Via Peroxisome Proliferator-Activated Receptor-γ Activation
Protein & Peptide Letters Roles of Adrenomedullin in Hypertension and Hypertensive Organ Damage
Current Hypertension Reviews Role of Chemokines and Their Receptors in Cancer
Current Pharmaceutical Design Chaperone-Mediated Autophagy: A Potential Target for Metabolic Diseases
Current Medicinal Chemistry Biomarkers of Alzheimer’s Disease: An Overview of the Recent Inventions
Recent Patents on Biomarkers Circumventing Melanoma Chemoresistance by Targeting DNA Repair
Current Medicinal Chemistry Inhibition of Type 1 Diabetes Development by Vitamin D Receptor Agonists
Current Medicinal Chemistry - Anti-Inflammatory & Anti-Allergy Agents Metabolic Remodeling Induced by Adipocytes: A New Achilles' Heel in Invasive Breast Cancer?
Current Medicinal Chemistry Statins, Risk of Death and Ischemic Stroke in Patients with Dementia: A Registry-Based Observational Cohort Study
Current Alzheimer Research Potential Therapeutic Applications of C-Phycocyanin
Current Drug Metabolism Cardiovascular Effects of Metabolic Surgery on Type 2 Diabetes
Current Cardiology Reviews Targeting Signal Transduction Pathways in the Treatment of Mood Disorders: Recent Insights into the Relevance of the Wnt Pathway
CNS & Neurological Disorders - Drug Targets Interrelationships between Hepatic Fat and Insulin Resistance in Non- Alcoholic Fatty Liver Disease
Current Diabetes Reviews Role of Autophagy in Parkinson’s Disease
Current Medicinal Chemistry Protective Effects of Caffeic Acid and the Alzheimer's Brain: An Update
Mini-Reviews in Medicinal Chemistry Formulation Strategies and Particle Engineering Technologies for Pulmonary Delivery of Biopharmaceuticals
Current Pharmaceutical Design