Abstract
In this study, a capillary electrophoresis-mass spectrometry method was applied to the determination of isoflavones in samples with low content in these analytes, such as legumes. Sample stacking was used to enhance the sensitivity of the technique. Extraction of the analytes was carried out by a modified approach of QuEChERS (Quick, Easy, Cheap, Effective, Rugged and Safe) methodology, in which the extracts were injected directly into electrophoretic system coupled with mass spectrometry detector. The developed method was validated and applied for the determination of isoflavone aglycones and glycosides in legume samples. The values of the validation parameters and the isoflavone contents found were compared with those obtained by LC-MS/MS. Both methods provided similar results and were comparable in terms of recovery (in the 80-120% range), precision (lower than 10 %) and limits of detection (ranging from 0.20 to 1.8 μg L-1).
Keywords: CE-ESI-MS, LC-ESI-MS/MS, Isoflavone, legume, QuEChERS.
Current Analytical Chemistry
Title:Determination of Isoflavones in Legumes by QuEChERS-Capillary Electrophoresis-Electrospray Ionization-Mass Spectrometry
Volume: 11 Issue: 2
Author(s): Lara Perez-Martin, Myriam Bustamante-Rangel and Maria Milagros Delgado-Zamarreno
Affiliation:
Keywords: CE-ESI-MS, LC-ESI-MS/MS, Isoflavone, legume, QuEChERS.
Abstract: In this study, a capillary electrophoresis-mass spectrometry method was applied to the determination of isoflavones in samples with low content in these analytes, such as legumes. Sample stacking was used to enhance the sensitivity of the technique. Extraction of the analytes was carried out by a modified approach of QuEChERS (Quick, Easy, Cheap, Effective, Rugged and Safe) methodology, in which the extracts were injected directly into electrophoretic system coupled with mass spectrometry detector. The developed method was validated and applied for the determination of isoflavone aglycones and glycosides in legume samples. The values of the validation parameters and the isoflavone contents found were compared with those obtained by LC-MS/MS. Both methods provided similar results and were comparable in terms of recovery (in the 80-120% range), precision (lower than 10 %) and limits of detection (ranging from 0.20 to 1.8 μg L-1).
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Cite this article as:
Perez-Martin Lara, Bustamante-Rangel Myriam and Delgado-Zamarreno Milagros Maria, Determination of Isoflavones in Legumes by QuEChERS-Capillary Electrophoresis-Electrospray Ionization-Mass Spectrometry, Current Analytical Chemistry 2015; 11 (2) . https://dx.doi.org/10.2174/1573411011999150112121608
DOI https://dx.doi.org/10.2174/1573411011999150112121608 |
Print ISSN 1573-4110 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-6727 |
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