Abstract
Phospholipids are the main constituents of biological membranes. Their amphiphilic character is responsible for the typical bilayer arrangement as the structural basis of biological membranes. In addition to their structural role, some phospholipids are of significant functional importance. They act as intercellular messengers and are involved in the pathogenesis of many diseases. Therefore, phospholipid research (“lipidomics”) has significantly advanced in the last decades, generating the need for fast, reliable, and informative analytical techniques. The aim of this review is to demonstrate the power of 31P nuclear magnetic resonance (NMR) spectroscopy for structural and analytical phospholipid research. High resolution 31P NMR provides information on the composition of phospholipid mixtures, whereas solid-state 31P NMR gives structural information about the sample phase and morphology. This review provides an introduction into the field and a short overview of currently used analytical and physicochemical methods to study these biomolecules. We will provide a theoretical description of 31P NMR spectroscopy and discuss methods for obtaining highly resolved phospholipid spectra. Selected applications of 31P NMR to aid phospholipid analysis and the investigation of phospholipid structures, membrane-peptide interactions, and enzyme activities are discussed. This review ends with an overview on 31P NMR applications to the analysis of body fluids, cells, and tissues.
Keywords: Lipidomics, Phospholipid phases, Detergents, High resolution and solid-state 31P NMR, Phospholipases, Lipid mixtures
Current Analytical Chemistry
Title: 31P NMR Spectroscopy of Phospholipids: From Micelles to Membranes
Volume: 3 Issue: 4
Author(s): Jurgen Schiller, Matthias Muller, Beate Fuchs, Klaus Arnold and Daniel Huster
Affiliation:
Keywords: Lipidomics, Phospholipid phases, Detergents, High resolution and solid-state 31P NMR, Phospholipases, Lipid mixtures
Abstract: Phospholipids are the main constituents of biological membranes. Their amphiphilic character is responsible for the typical bilayer arrangement as the structural basis of biological membranes. In addition to their structural role, some phospholipids are of significant functional importance. They act as intercellular messengers and are involved in the pathogenesis of many diseases. Therefore, phospholipid research (“lipidomics”) has significantly advanced in the last decades, generating the need for fast, reliable, and informative analytical techniques. The aim of this review is to demonstrate the power of 31P nuclear magnetic resonance (NMR) spectroscopy for structural and analytical phospholipid research. High resolution 31P NMR provides information on the composition of phospholipid mixtures, whereas solid-state 31P NMR gives structural information about the sample phase and morphology. This review provides an introduction into the field and a short overview of currently used analytical and physicochemical methods to study these biomolecules. We will provide a theoretical description of 31P NMR spectroscopy and discuss methods for obtaining highly resolved phospholipid spectra. Selected applications of 31P NMR to aid phospholipid analysis and the investigation of phospholipid structures, membrane-peptide interactions, and enzyme activities are discussed. This review ends with an overview on 31P NMR applications to the analysis of body fluids, cells, and tissues.
Export Options
About this article
Cite this article as:
Schiller Jurgen, Muller Matthias, Fuchs Beate, Arnold Klaus and Huster Daniel, 31P NMR Spectroscopy of Phospholipids: From Micelles to Membranes, Current Analytical Chemistry 2007; 3 (4) . https://dx.doi.org/10.2174/157341107782109635
DOI https://dx.doi.org/10.2174/157341107782109635 |
Print ISSN 1573-4110 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-6727 |
Call for Papers in Thematic Issues
Advancements in biomedicine, corrosion inhibition/monitoring, and lubrication of carbon dots
Carbon dots, a class of carbon-based nanomaterials, have garnered substantial interest in various scientific and engineering fields due to their unique properties, including excellent optical properties, outstanding biocompatibility, easy preparation, good dispersity, ultrasmall size, low toxicity and environmental friendliness. This special issue of Current Analytical Chemistry aims to showcase cutting-edge ...read more
Advances in Microfluidic and Sensing Technologies of Biofluids.
Detection of biological fluids is an important means to assess human health and diagnose diseases. Currently, research on blood is relatively mature and widely applied in the medical field. With the development and advancement of microfluidic technology and novel detection methods, attention has turned to human physiological fluids such as ...read more
Analytical methods for environmental and food analysis
In recent years, the study of the chemical composition of food and the environment depends on analytical methods in order to acquire information about the chemical composition, processing, quality control and contamination of soil, water, air, plants and foods, aspects very important for food safety and environmental quality control. There ...read more
Applications of artificial intelligence in chemical analysis
Artificial intelligence (AI) has demonstrated its versatility in almost all scientific fields, especially analytical chemistry, which is leading the way in the integration of AI. According to Chemical Abstract Services, one of the major disciplines contributing to AI-related publications and patents is analytical chemistry. That?s because techniques such as deep ...read more
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
Related Articles
-
Recent Developments in the Mechanism of Anticancer Agents Based on Electron Transfer, Reactive Oxygen Species and Oxidative Stress
Anti-Cancer Agents in Medicinal Chemistry Targeting Cyclooxygenase-2 in Hematological Malignancies: Rationale and Promise
Current Pharmaceutical Design Targeting Stim and Orai Proteins as an Alternative Approach in Anticancer Therapy
Current Medicinal Chemistry Macrocyclic Inhibitors of Hsp90
Current Topics in Medicinal Chemistry Curcumin Targets in Inflammation and Cancer
Endocrine, Metabolic & Immune Disorders - Drug Targets Structurally Modified ‘Dietary Flavonoids’: Are these Viable Drug Candidates for Chemoprevention?
Current Clinical Pharmacology Identification of Novel Anti-inflammatory Agents from Ayurvedic Medicine for Prevention of Chronic Diseases: “Reverse Pharmacology” and “Bedside to Bench” Approach
Current Drug Targets The Urokinase Receptor as a Potential Target in Cancer Therapy
Current Pharmaceutical Design Genetic Variants in Genes Involved in Mechanisms of Chemoresistance to Anticancer Drugs
Current Cancer Drug Targets Advances in Synergistic Combinations of Chinese Herbal Medicine for the Treatment of Cancer
Current Cancer Drug Targets Cancer Stem Cell Model in Oral Squamous Cell Carcinoma
Current Stem Cell Research & Therapy Combining Gene Therapy and Radiation Against Cancer
Current Gene Therapy Vitamin D: Evolutionary, Physiological and Health Perspectives
Current Drug Targets Cytomegalovirus Infection in Pediatric Immunocompromised Hosts
Infectious Disorders - Drug Targets Towards Tyrosine Metabolism in Esophageal Squamous Cell Carcinoma
Combinatorial Chemistry & High Throughput Screening Right Heart Catheterization Through Persistent Left Superior Vena Cava, an Extremely Rare Procedure and Review of Current Literature
Current Cardiology Reviews Adverse Effects of Statins - Myths and Reality
Current Pharmaceutical Design Inventions Designed to Enhance Drug Delivery Across Epithelial and Endothelial Cells Through the Paracellular Pathway
Recent Patents on Drug Delivery & Formulation E-Cadherin Upregulation as a Therapeutic Goal in Cancer Treatment
Mini-Reviews in Medicinal Chemistry Bile Acids and Derivatives, Their Nuclear Receptors FXR, PXR and Ligands: Role in Health and Disease and Their Therapeutic Potential
Anti-Cancer Agents in Medicinal Chemistry