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Recent Patents on Anti-Cancer Drug Discovery

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ISSN (Print): 1574-8928
ISSN (Online): 2212-3970

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Research Article

The Effect of Acetylsalicylic Acid (Asa) on the Mechanical Properties of Breast Cancer Epithelial Cells

Author(s): Dornaz Milani, Siamak Khorramymehr* and Behnoush Vasaghi-Gharamaleki

Volume 17, Issue 4, 2022

Published on: 07 March, 2022

Page: [410 - 415] Pages: 6

DOI: 10.2174/1574892817666220104094846

Price: $65

Abstract

Background: In most communities, the risk of developing breast cancer is increasing. By affecting the cyclooxygenase 1 and 2 (COX-1 and COX-2) enzymes and actin filaments, acetylsalicylic acid (Aspirin) has been shown to reduce the risk of breast cancer and prevent cell migration in both laboratory and clinical studies.

Methods: The purpose of this study is to determine the mechanical properties of normal and cancerous breast tissue cells, as well as the short-term effect of aspirin on cancer cells. To this end, the mechanical properties and deformation of three cell types were investigated: healthy MCF-10 breast cells, MCF-7 breast cancer cells, and MCF-7 breast cancer cells treated with a 5 μM aspirin solution. Atomic Force Microscopy (AFM) was used to determine the mechanical properties of the cells. Cell deformation was analyzed in all groups, and Young's modulus was calculated using the Hertz model.

Results: According to the obtained data, cancer cells deformed at a rate half that of healthy cells. Nonetheless, when aspirin was used, cancer cells deformed similarly to healthy cells. Additionally, healthy cells' Young's modulus was calculated to be approximately three times that of cancer cells, which was placed closer to that of healthy cells by adding aspirin to Young's modulus.

Conclusion: Cell strength appears to have increased due to aspirin's intervention on actin filaments and cytoskeletons, and the mechanical properties of breast cancer cells have become more similar to those of normal cells. The likelihood of cell migration and metastasis decreases as cell strength increases.

Keywords: Cell mechanics, cancer cell, acetylsalicylic acid (ASA), atomic force microscopy, breast cancer, cancer drug.

« Previous Next »
[1]
DeSantis CE, Ma J, Gaudet MM, et al. Breast cancer statistics, 2019. CA Cancer J Clin 2019; 69(6): 438-51.
[http://dx.doi.org/10.3322/caac.21583] [PMID: 31577379]
[2]
Malvezzi M, Carioli G, Bertuccio P, et al. European cancer mortality predictions for the year 2019 with focus on breast cancer. Ann Oncol 2019; 30(5): 781-7.
[http://dx.doi.org/10.1093/annonc/mdz051] [PMID: 30887043]
[3]
Faridi N, Bathaie SZ, Abroun S, et al. Isolation and characterization of the primary epithelial breast cancer cells and the adjacent normal epithelial cells from Iranian women’s breast cancer tumors. Cytotechnology 2018; 70(2): 625-39.
[http://dx.doi.org/10.1007/s10616-017-0159-3] [PMID: 29380298]
[4]
Lee GYH, Lim CT. Biomechanics approaches to studying human diseases. Trends Biotechnol 2007; 25(3): 111-8.
[http://dx.doi.org/10.1016/j.tibtech.2007.01.005] [PMID: 17257698]
[5]
Chen WY, Holmes MD. Role of aspirin in breast cancer survival. Curr Oncol Rep 2017; 19(7): 48.
[http://dx.doi.org/10.1007/s11912-017-0605-6] [PMID: 28597105]
[6]
Dikshit P, Chatterjee M, Goswami A, Mishra A, Jana NR. Aspirin induces apoptosis through the inhibition of proteasome function. J Biol Chem 2006; 281(39): 29228-35.
[http://dx.doi.org/10.1074/jbc.M602629200] [PMID: 16880202]
[7]
Thottan MK, Chuanyi J. Patent Application Publication. US 2004/ 0231417 A1, 2004; 1(19): 12-7.
[8]
Clarke CA, Canchola AJ, Moy LM, et al. Regular and low-dose aspirin, other non-steroidal anti-inflammatory medications and prospective risk of HER2-defined breast cancer: The California Teachers Study. Breast Cancer Res 2017; 19(1): 52.
[http://dx.doi.org/10.1186/s13058-017-0840-7] [PMID: 28460643]
[9]
Wodarz D, Goel A, Boland CR, Komarova NL. Effect of aspirin on tumour cell colony formation and evolution. Journal of the Royal Society 2017; 14(134): 20170374.
[10]
Wong RUSY. Role of Nonsteroidal Anti-Inflammatory Drugs ( NSAIDs ) in cancer prevention and cancer promotion. Pharmacol 2019; 2019: 341897.
[11]
Amaral MEA, Nery LR, Leite CE, de Azevedo Junior WF, Campos MM. Pre-clinical effects of metformin and aspirin on the cell lines of different breast cancer subtypes. Invest New Drugs 2018; 36(5): 782-96.
[http://dx.doi.org/10.1007/s10637-018-0568-y] [PMID: 29392539]
[12]
Liggett JL, Zhang X, Eling TE, Baek SJ. Anti-tumor activity of non-steroidal anti-inflammatory drugs: Cyclooxygenase-independent targets. Cancer Letters 2014; 346(2): 217-24.
[13]
Hu LX, Du YY, Zhang Y, Pan YY. Synergistic effects of exemestane and aspirin on MCF-7 human breast cancer cells. Asian Pac J Cancer Prev 2012; 13(11): 5903-8.
[http://dx.doi.org/10.7314/APJCP.2012.13.11.5903] [PMID: 23317278]
[14]
Gurpinar E, Grizzle WE, Piazza GA. NSAIDs inhibit tumorigenesis, but how? Clin Cancer Res 2014; 20(5): 1104-13.
[http://dx.doi.org/10.1158/1078-0432.CCR-13-1573] [PMID: 24311630]
[15]
Variable I, Tal S, Garcia T. United States Patent 2011; 2(12): 1849-60.
[16]
Abu M, Muhamad M, Hassan H, Zakaria Z, Ali SAM. Proximity coupled antenna with star geometry pattern amc ground plane. J Eng Appl Sci (Asian Res Publ Netw) 2016; 11(14): 8822-8.
[17]
Zuckerman LM, Frames WL, Elsissy JG, et al. The effect of non-steroidal anti-inflammatory drugs on osteosarcoma cells. Eur Rev Med Pharmacol Sci 2019; 23(6): 2681-90.
[PMID: 30964195]
[18]
Hiľovská L, Jendželovský R, Fedoročko P. Potency of non-steroidal anti-inflammatory drugs in chemotherapy. Mol Clin Oncol 2015; 3(1): 3-12.
[http://dx.doi.org/10.3892/mco.2014.446] [PMID: 25469262]
[19]
Moris D, Kontos M, Spartalis E, Fentiman IS. The role of NSAIDs in breast cancer prevention and relapse: current evidence and future perspectives. Breast Care (Basel) 2016; 11(5): 339-44.
[http://dx.doi.org/10.1159/000452315] [PMID: 27920627]
[20]
Kirmizis D, Logothetidis S. Atomic force microscopy probing in the measurement of cell mechanics. Int J Nanomedicine 2010; 5(1): 137-45.
[http://dx.doi.org/10.2147/IJN.S5787] [PMID: 20463929]
[21]
Prabhune M, Belge G, Dotzauer A, Bullerdiek J, Radmacher M. Comparison of mechanical properties of normal and malignant thyroid cells. Micron 2012; 43(12): 1267-72.
[http://dx.doi.org/10.1016/j.micron.2012.03.023] [PMID: 22522060]
[22]
Lee MH, Wu PH, Staunton JR, Ros R, Longmore GD, Wirtz D. Mismatch in mechanical and adhesive properties induces pulsating cancer cell migration in epithelial monolayer. Biophys J 2012; 102(12): 2731-41.
[http://dx.doi.org/10.1016/j.bpj.2012.05.005] [PMID: 22735523]
[23]
Guo X, Bonin K, Scarpinato K, Guthold M. The effect of neighboring cells on the stiffness of cancerous and non-cancerous human mammary epithelial cells. New J Phys 2015; 21(15): 057004.
[http://dx.doi.org/10.1088/1367-2630/16/10/105002]
[24]
Suresh S, Spatz J, Mills JP, et al. Connections between single-cell biomechanics and human disease states: Gastrointestinal cancer and malaria. Acta Biomater 2005; 1(1): 15-30.
[http://dx.doi.org/10.1016/j.actbio.2004.09.001] [PMID: 16701777]
[25]
Cross SE, Jin YS, Tondre J, Wong R, Rao J, Gimzewski JK. AFM-based analysis of human metastatic cancer cells. Nanotechnology 2008; 19(38): 384003.
[http://dx.doi.org/10.1088/0957-4484/19/38/384003] [PMID: 21832563]
[26]
Suresh S. Biomechanics and biophysics of cancer cells. Acta Biomater 2007; 3(4): 413-38.
[http://dx.doi.org/10.1016/j.actbio.2007.04.002] [PMID: 17540628]
[27]
Swaminathan V, Mythreye K, O’Brien ET, Berchuck A, Blobe GC, Superfine R. Mechanical stiffness grades metastatic potential in patient tumor cells and in cancer cell lines. Cancer Res 2011; 71(15): 5075-80.
[http://dx.doi.org/10.1158/0008-5472.CAN-11-0247] [PMID: 21642375]
[28]
Cross SE, Jin YS, Rao J, Gimzewski JK. Nanomechanical analysis of cells from cancer patients. Nat Nanotechnol 2007; 2(12): 780-3.
[http://dx.doi.org/10.1038/nnano.2007.388] [PMID: 18654431]
[29]
Hu S, Lam RHW. Characterization of viscoelastic properties of normal and cancerous human breast cells using a confining microchannel. Microfluid Nanofluidics 2017; 21(68)
[http://dx.doi.org/10.1007/s10404-017-1903-x]
[30]
Coughlin MF, Bielenberg DR, Lenormand G, et al. Cytoskeletal stiffness, friction, and fluidity of cancer cell lines with different metastatic potential. Clin Exp Metastasis 2013; 30(3): 237-50.
[http://dx.doi.org/10.1007/s10585-012-9531-z] [PMID: 22961212]
[31]
Schierbaum N, Rheinlaender J, Schäffer TE. Viscoelastic properties of normal and cancerous human breast cells are affected differently by contact to adjacent cells. Acta Biomater 2017; 55: 239-48.
[http://dx.doi.org/10.1016/j.actbio.2017.04.006] [PMID: 28396292]
[32]
Heydarian A, Khorramymehr S, Vasaghi-Gharamaleki B. Short-term effects of X-ray on viscoelastic properties of epithelial cells. Proc Inst Mech Eng H 2019; 233(5): 535-43.
[http://dx.doi.org/10.1177/0954411919837563] [PMID: 30919725]
[33]
Heydarian A, Milani D, Moein Fatemi SM. An investigation of the viscoelastic behavior of MCF-10A and MCF-7 cells. Biochem Biophys Res Commun 2020; 529(2): 432-6.
[http://dx.doi.org/10.1016/j.bbrc.2020.06.010] [PMID: 32703447]
[34]
Guck J, Schinkinger S, Lincoln B, et al. Optical deformability as an inherent cell marker for testing malignant transformation and metastatic competence. Biophys J 2005; 88(5): 3689-98.
[http://dx.doi.org/10.1529/biophysj.104.045476] [PMID: 15722433]
[35]
Yasaman N, Kuin Tian P, Chwee Teck L. Correlating the viscoelasticity of breast cancer cells with their malignancy. Converg Sci Phys Oncol 2017; 3(034003): 1-13.
[36]
Katira P, Bonnecaze RT, Zaman MH. Modeling the mechanics of cancer: Effect of changes in cellular and extra-cellular mechanical properties. Front Oncol 2013; 3: 145.
[http://dx.doi.org/10.3389/fonc.2013.00145] [PMID: 23781492]
[37]
Li Q, Mattingly RR. Restoration of E-cadherin cell-cell junctions requires both expression of E-cadherin and suppression of ERK MAP kinase activation in Ras-transformed breast epithelial cells. Neoplasia 2008; 10(12): 1444-58.
[http://dx.doi.org/10.1593/neo.08968] [PMID: 19048123]
[38]
Rosenbluth MJ, Lam WA, Fletcher DA. Force microscopy of nonadherent cells: A comparison of leukemia cell deformability. Biophys J 2006; 90(8): 2994-3003.
[http://dx.doi.org/10.1529/biophysj.105.067496] [PMID: 16443660]
[39]
Zhang T, Zhao Y, Tong Z, Guan Y. A novel method to calculate the mechanical properties of cancer cells based on atomic force microscopy. Acta Bioeng Biomech 2016; 18(1): 19-24.
[PMID: 27151360]
[40]
Kámán J. Young’s Modulus and Energy Dissipation Determination Methods by AFM, with Particular Reference to a Chalcogenide Thin Film. Period Polytech Electr Eng Comput Sci 2015; 59(1): 18-25.
[http://dx.doi.org/10.3311/PPee.7865]
[41]
Li QS, Lee GYH, Ong CN, Lim CT. AFM indentation study of breast cancer cells. Biochem Biophys Res Commun 2008; 374(4): 609-13.
[http://dx.doi.org/10.1016/j.bbrc.2008.07.078] [PMID: 18656442]
[42]
Omidvar R, Tafazzoli-Shadpour M, Shokrgozar MA, Rostami M. Atomic force microscope-based single cell force spectroscopy of breast cancer cell lines: An approach for evaluating cellular invasion. J Biomech 2014; 47(13): 3373-9.
[http://dx.doi.org/10.1016/j.jbiomech.2014.08.002] [PMID: 25169659]
[43]
Lekka M. Discrimination between normal and cancerous cells using AFM. Bionanoscience 2016; 6(1): 65-80.
[http://dx.doi.org/10.1007/s12668-016-0191-3] [PMID: 27014560]
[44]
Deng X, Xiong F, Li X, et al. Application of atomic force microscopy in cancer research. J Nanobiotechnology 2018; 16(1): 102.
[http://dx.doi.org/10.1186/s12951-018-0428-0] [PMID: 30538002]
[45]
Melzak KA, Toca-Herrera JL. Atomic force microscopy and cells: Indentation profiles around the AFM tip, cell shape changes, and other examples of experimental factors affecting modeling. Microsc Res Tech 2015; 78(7): 626-32.
[http://dx.doi.org/10.1002/jemt.22522] [PMID: 26031471]
[46]
Lin DC, Dimitriadis EK, Horkay F. Robust strategies for automated AFM force curve analysis- I. Non-adhesive indentation of soft, inhomogeneous materials. J Biomech Eng 2007; 129(3): 430-40.
[http://dx.doi.org/10.1115/1.2720924] [PMID: 17536911]
[47]
Lekka M, Laidler P, Gil D, Lekki J, Stachura Z, Hrynkiewicz AZ. Elasticity of normal and cancerous human bladder cells studied by scanning force microscopy. Eur Biophys J 1999; 28(4): 312-6.
[http://dx.doi.org/10.1007/s002490050213] [PMID: 10394623]
[48]
Lloyd FP Jr, Slivova V, Valachovicova T, Sliva D. Aspirin inhibits highly invasive prostate cancer cells. Int J Oncol 2003; 23(5): 1277-83.
[http://dx.doi.org/10.3892/ijo.23.5.1277] [PMID: 14532966]
[49]
López-Contreras L, Hernández-Ramírez VI, Lagunes-Guillén AE, et al. Exploring the possible role of lysine acetylation on Entamoeba histolytica virulence: A focus on the dynamics of the actin cytoskeleton. BioMed Res Int 2013; 2013: 757392.
[PMID: 24078923]

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