Abstract
A fluorescent probe is a fluorophore designed to localize within a specific region of a biological specimen or to respond to a specific stimulus. Fluorescent probes have been used for nearly a century to study cellular processes due to their exquisite sensitivity and selectivity. Fluorescent probes have also gained in popularity as safety and environmental concerns over the use of radioactive probes have grown. At the same time, cellular assays are being more widely used now than ever before. This review will give a broad overview of types of fluorescent probes, types of fluorescent assays, and their application in cellular assays for a number of pharmaceutically relevant target classes.
Keywords: Fluorescence, cell based assays, high throughput screening, GPCR, pathway, ion channel, kinase, multiplexing.
Combinatorial Chemistry & High Throughput Screening
Title: Fluorescent Probes for Cellular Assays
Volume: 11 Issue: 7
Author(s): George T. Hanson and Bonnie J. Hanson*
Affiliation:
- Invitrogen Discovery Sciences, 501 Charmany Drive, Madison, WI 53719, USA.,United States
Keywords: Fluorescence, cell based assays, high throughput screening, GPCR, pathway, ion channel, kinase, multiplexing.
Abstract: A fluorescent probe is a fluorophore designed to localize within a specific region of a biological specimen or to respond to a specific stimulus. Fluorescent probes have been used for nearly a century to study cellular processes due to their exquisite sensitivity and selectivity. Fluorescent probes have also gained in popularity as safety and environmental concerns over the use of radioactive probes have grown. At the same time, cellular assays are being more widely used now than ever before. This review will give a broad overview of types of fluorescent probes, types of fluorescent assays, and their application in cellular assays for a number of pharmaceutically relevant target classes.
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Cite this article as:
Hanson T. George and Hanson J. Bonnie*, Fluorescent Probes for Cellular Assays, Combinatorial Chemistry & High Throughput Screening 2008; 11 (7) . https://dx.doi.org/10.2174/138620708785204090
DOI https://dx.doi.org/10.2174/138620708785204090 |
Print ISSN 1386-2073 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5402 |
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