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Current Functional Foods

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ISSN (Print): 2666-8629
ISSN (Online): 2666-8637

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Research Article

Exploration and Evaluation of In-vitro Antioxidant Activity of ABANA: A Polyherbal Formulation

Author(s): Deepak Bharati* and Abhijeet Puri

Volume 1, Issue 2, 2023

Published on: 03 September, 2022

Article ID: e060622205665 Pages: 10

DOI: 10.2174/2666862901666220606155358

Abstract

Objective: The purpose of this study was to determine the antioxidant capacity of Abana ® (a poly-ingredient formulation with natural constituents).

Methods: Extensive ethanol extraction of polyherbal formulations was followed by fractionation into petroleum ether, chloroform, and ethyl acetate extracts. Various fractions were further analyzed using this conventional method for phytochemical composition and concentration-dependent antioxidant activity. Due to the presence of phenolic compounds in the formulation, the extracts were analyzed for total phenolic content, flavonoid content, and in-vitro antioxidant activity. Antioxidant capacity was determined using total reducing potential, 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2-azinobis-ethyl-benzothiazoline-sulphonic acid diammonium salt (ABTS) and nitric oxide inhibition assays.

Results: Primitive phytochemical screening revealed the presence of steroids, saponins, flavonoids, alkaloids, and tannins which were confirmed using thin-layer chromatography. The antioxidant activity of Abana tablet extracts decreased in the order of ethyl acetate > ethanolic > chloroform > petroleum ether comparable to that of ascorbic acid and butylated hydroxytoluene.

Conclusion: The current investigation suggests that the ethanolic extract and fractions of Abana polyherbal formulations exhibit significant antioxidant activity. Our results strongly imply that polyherbal compounds are a potential source of antioxidants capable of scavenging free radicals. The strong positive connection between antiradical scavenging activity and overall polyphenolic concentration in polyherbal drugs demonstrates that polyphenols are significant components capable of scavenging free radicals. Additional research is required to isolate and characterize the active moiety responsible for biological activity and treat it under various stress conditions.

Keywords: Abana, DPPH, ABTS, antioxidant, free radical scavenging, polyherbal formulation.

Graphical Abstract

[1]
Ak G, Zengin G, Ceylan R, et al. Chemical composition and biological activities of essential oils from Calendula officinalis L. flowers and leaves. Flavour Fragrance J 2021; 36(5): 554-63.
[http://dx.doi.org/10.1002/ffj.3661]
[2]
Ekor M. The growing use of herbal medicines: Issues relating to adverse reactions and challenges in monitoring safety. Front Pharmacol 2014; 4: 177-7.
[http://dx.doi.org/10.3389/fphar.2013.00177] [PMID: 24454289]
[3]
Yuan H, Ma Q, Ye L, Piao G. The traditional medicine and modern medicine from natural products. Molecules 2016; 21(5): 559.
[http://dx.doi.org/10.3390/molecules21050559] [PMID: 27136524]
[4]
Sharma S, Sharma S, Pradhan P, Pathak S, Sharma M. Development, standardization of polyherbal formulation of analgesic ointment of plant Carum copticum, Mentha piperita, Cedrus deodara. J Appl Pharm Res 2020; 8(1): 29-43.
[http://dx.doi.org/10.18231/j.joapr.2019.v.8.i.1.004]
[5]
Yang W, Zhang Y, Wu W, Huang L, Guo D, Liu C. Approaches to establish Q-markers for the quality standards of traditional Chinese medicines. Acta Pharm Sin B 2017; 7(4): 439-46.
[http://dx.doi.org/10.1016/j.apsb.2017.04.012] [PMID: 28752028]
[6]
Barik CS, Kanungo SK, Tripathy NK, Panda JR, Padhi M. A review on therapeutic potential of polyherbal formulations. Int J Pharm Sci Drug Res 2015; 7(3)
[7]
Kajaria DK, Gangwar M, Sharma AK, Tripathi YB, Tripathi JS, Tiwari S. Evaluation of in vitro antioxidant capacity and reducing potential of polyherbal drug- bhāraṅgyādi. Anc Sci Life 2012; 32(1): 24-8.
[http://dx.doi.org/10.4103/0257-7941.113798] [PMID: 23929990]
[8]
Puri A. Quantitative phytochemical screening, thin-layer chromatography analysis, high-performance thin-layer chromatography fingerprinting, and antioxidant activity of leaves of diospyros montana. Asian J Pharm Clin Res 2019; 12(2): 325-31.
[9]
Vidyashankar S, Godavarthi A, Varma RS, Nandakumar KS. Water-soluble compounds in the herbal preparation Abana inhibit lipid biosynthesis and enhance cholesterol efflux in HepG2 cells. Can J Physiol Pharmacol 2010; 88(4): 456-64.
[http://dx.doi.org/10.1139/Y10-021] [PMID: 20555414]
[10]
Mani V, Parle M. Evidence of anti-demential potential of Abana®: An indian ayurvedic poly-herbal formulation in rats. Pharmacologyonline 2007; 1.
[11]
Rafiq M, Azeemuddin M, Rajesh S, et al. Anti-platelet and anti-thrombotic effects of a poly-ingredient formulation: In vitro and in vivo experimental evidences. Oman Med J 2012; 27(6): e010-0.
[PMID: 28804574]
[12]
Mocan A, Zengin G, Simirgiotis M, et al. Functional constituents of wild and cultivated Goji (L. barbarum L.) leaves: Phytochemical characterization, biological profile, and computational studies. J Enzyme Inhib Med Chem 2017; 32(1): 153-68.
[http://dx.doi.org/10.1080/14756366.2016.1243535] [PMID: 28095717]
[13]
Bharati D, Rawat S, Sharma P, Shrivastava B. Evaluation of in vivo efficacy of aqueous extract of aerial parts of cynodon dactylon in rats with simultaneous type 2 diabetes and hypertension. Curr Bioact Compd 2016; 12(1): 25-33.
[http://dx.doi.org/10.2174/1573407212666160210230059]
[14]
Puri A, Ansari Y, Priti P. Pharmacognostic, physicochemical and phytochemical investigation of leaves of Duranta repens linn. J Pharmacogn Phytochem 2017; 9: 141-6.
[http://dx.doi.org/10.5958/0975-4385.2017.00026.7]
[15]
Zhang Q-W, Lin L-G, Ye W-C. Techniques for extraction and isolation of natural products: A comprehensive review. Chin Med 2018; 13: 20-0.
[http://dx.doi.org/10.1186/s13020-018-0177-x] [PMID: 29692864]
[16]
Puri A, Akki KS, Savadi RV, Manjunath KP. Preliminary phytochemical investigation and free radical scavenging activity of Diospyros montana Roxburg. leaves. Indian Drugs 2010; 47: 48-52.
[17]
Puri A. Microscopical evaluation, phytochemical screening, and high-performance thin-layer chromatography fingerprinting of leaves of Diospyros montana. Int J Green Pharm 2018; 12: 205-11.
[18]
Fawzi MM, Mollica A, Stefanucci A, Zakariyyah AM, Poorneeka R, Zengin G. Volatile components, pharmacological profile, and computational studies of essential oil from Aegle marmelos (Bael) leaves: A functional approach. Ind Crops Prod 2018; 126: 13-21.
[http://dx.doi.org/10.1016/j.indcrop.2018.09.054]
[19]
Zengin G, Locatelli M, Stefanucci A, et al. Chemical characterization, antioxidant properties, anti-inflammatory activity, and enzyme inhibition of Ipomoea batatas L. leaf extracts. Int J Food Prop 2017; 20(sup2): 1907-19.
[20]
Rahim NFA, Muhammad N, Abdullah N, Talip BHA, Dusuki NJS. Polyherbal formulations with optimum antioxidant properties. AIP Conf Proc 2018; 2016(1): 020007.
[http://dx.doi.org/10.1063/1.5055409]
[21]
Wakkumbura HP, Wickramaarachchi WMD, Arawwawala LDAM, Liyanage JA, Rajapakse RPVJ. Assessment of the quality and evaluation of the antioxidant potential of a novel Srilankan ayurvedic polyherbal formulation. Evid Based Complement Alternat Med 2020; 20202319315
[http://dx.doi.org/10.1155/2020/2319315] [PMID: 32733579]
[22]
Adebayo SA, Ondua M, Shai LJ, Lebelo SL. Inhibition of nitric oxide production and free radical scavenging activities of four South African medicinal plants. J Inflamm Res 2019; 12: 195-203.
[http://dx.doi.org/10.2147/JIR.S199377] [PMID: 31496781]
[23]
Zhang Y-J, Gan R-Y, Li S, et al. Antioxidant phytochemicals for the prevention and treatment of chronic diseases. Molecules 2015; 20(12): 21138-56.
[http://dx.doi.org/10.3390/molecules201219753] [PMID: 26633317]
[24]
Tungmunnithum D, Thongboonyou A, Pholboon A, Yangsabai A. Flavonoids and other phenolic compounds from medicinal plants for pharmaceutical and medical aspects: An overview. Medicines (Basel) 2018; 5(3): 93.
[http://dx.doi.org/10.3390/medicines5030093] [PMID: 30149600]
[25]
Shahidi F, Ambigaipalan P. Phenolics and polyphenolics in foods, beverages and spices: Antioxidant activity and health effects – A review. J Funct Foods 2015; 18: 820-97.
[http://dx.doi.org/10.1016/j.jff.2015.06.018]
[26]
Chanthasri W, Puangkeaw N, Kunworarath N, et al. Antioxidant capacities and total phenolic contents of 20 polyherbal remedies used as tonics by folk healers in Phatthalung and Songkhla provinces, Thailand. BMC Complement Altern Med 2018; 18(1): 73-3.
[http://dx.doi.org/10.1186/s12906-018-2131-y] [PMID: 29466987]
[27]
Apak R, Özyürek M, Güçlü K. Çapanoğlu E. Antioxidant activity/capacity measurement. 1. classification, physicochemical principles, mechanisms, and electron transfer (et)-based assays. J Agric Food Chem 2016; 64(5): 997-1027.
[http://dx.doi.org/10.1021/acs.jafc.5b04739] [PMID: 26728425]
[28]
Baranowska M, Koziara Z, Suliborska K, et al. Interactions between polyphenolic antioxidants quercetin and naringenin dictate the distinctive redox-related chemical and biological behaviour of their mixtures. Sci Rep 2021; 11(1): 12282.
[http://dx.doi.org/10.1038/s41598-021-89314-0] [PMID: 34112813]
[29]
Rahman MM, Islam MB, Biswas M, Khurshid Alam AHM. In vitro antioxidant and free radical scavenging activity of different parts of Tabebuia pallida growing in Bangladesh. BMC Res Notes 2015; 8(1): 621.
[http://dx.doi.org/10.1186/s13104-015-1618-6] [PMID: 26518275]
[30]
Saini R, Mishra A, Mishra S, Nathtiwari K, Kavindra N, Tiwari K. Evaluation of polyphenolic content and anti-oxidant potential of poly-herbal formulation. Int J Emerg Technol 2019; 10(2): 243-8.
[31]
Krumova K, Cosa G. Overview of reactive oxygen species. Singlet Oxygen: Applications in Biosciences and Nanosciences. Royal Society Chem 2016; 1: 1-21.
[32]
Sharifi-Rad M, Anil Kumar NV, Zucca P, et al. Lifestyle, oxidative stress, and antioxidants: Back and forth in the pathophysiology of chronic diseases. Front Physiol 2020; 11: 694.
[http://dx.doi.org/10.3389/fphys.2020.00694] [PMID: 32714204]
[33]
Ronzio RA. Naturally Occurring Antioxidants. Textbook of Natural Medicine. Fifth. Churchill Livingstone: St. Louis (MO) 2020; pp. 731-751.

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