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Nanoscience & Nanotechnology-Asia

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ISSN (Print): 2210-6812
ISSN (Online): 2210-6820

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Research Article

Silver Ion Release from Polymethylmethacrylate Reinforced with Silvernanoparticles-An In vitro Study

Author(s): Aashritha Shenava*

Volume 13, Issue 3, 2023

Published on: 25 May, 2023

Article ID: e130423215740 Pages: 8

DOI: 10.2174/2210681213666230413090403

Price: $65

Abstract

Background: Despite the awareness among patients with complete dentures, the risk factor for developing denture related candidiasis is when it is not removed during sleep and cleaned regularly. The routine treatment is an antifungal application, but frequent infection is seen after treatment. Nanotechnology has led the medical field to a cutting edge in most of the treatment aspects of various conditions. Moreover, the assimilation of silver nanoparticles into the polymer can be useful as an antifungal agent. To assess the antifungal efficacy of AgNP’s/ PMMA (silver nanoparticles /polymethlmethacrylate) against Candida albicansstrain and to evaluate the continuous release of silver ions which would increase antifungal mechanism.

Methods: Chemical methods of synthesis of silver nanoparticles using metal precursors, reducing agents, and stabilizing agents were used because of its convenience and simple equipment. MIC (minimum inhibitory concentration) was evaluated along with antifungal efficacy by incorporating PMMA (polymethylmethacrylate) discs with silver nanoparticles. Silver ion release was carried out by immersing the PMMA/Ag discs in deionised water for different immersion periods.

Results: In the Independent Sample ‘t’ test, the comparison of optical density between the study groups at 6 months at absorbance A230, A260,A280, A320, A420, and A550 was statistically significant (p < 0.05).

Conclusion: Silver Nanoparticles could be prepared which is cost-effective and can serve as an antifungal agent against Candida albicans. Silver ion release was seen with AgNP’s /PMMA (SilverNanoparticle /polymethylmethacrylate) at 6 months and Group A (.06M) and Group B (.03 M) was confirmed to be used as an antifungal agent.

Keywords: Silver ion release, polymethylmethacrylate, silver nanoparticles, Candida albicans, antifungal, agent, minimum fungicide, concentration.

Graphical Abstract

[1]
Kaviya, N.E.; Somasundaram, D.; Roy, D. Advancement in nanotechnology for restorative dentistry. Eur. J. Mol. Clin. Med., 2020, 7(1), 3295-3306.
[2]
Daniluk, T.; Tokajuk, G.; Stokowska, W.; Fiedoruk, K.; Sciepuk, M.; Zaremba, M.L.; Rozkiewicz, D.; Cylwik-Rokicka, D.; Kedra, B.A.; Anielska, I.; Górska, M.; Kedra, B.R. Occurrence rate of oral Candida albicans in denture wearer patients. Adv. Med. Sci., 2006, 51(Suppl. 1), 77-80.
[PMID: 17458064]
[3]
Fisher, B.M.; Lamey, P-J.; Samaranayake, L.P.; MacFarlane, T.W.; Frier, B.M. Carriage of Candida species in the oral cavity in diabetic patients: Relationship to glycaemic control. J. Oral Pathol. Med., 1987, 16(5), 282-284.
[http://dx.doi.org/10.1111/j.1600-0714.1987.tb01494.x] [PMID: 3116189]
[4]
Geerlings, S.E.; Hoepelman, A.I.M. Immune dysfunction in patients with diabetes mellitus (DM). FEMS Immunol. Med. Microbiol., 1999, 26(3-4), 259-265.
[http://dx.doi.org/10.1111/j.1574-695X.1999.tb01397.x] [PMID: 10575137]
[5]
Kumar, B.V.; Padshetty, N.S.; Bai, K.Y.; Rao, M.S. Prevalence of Candida in the oral cavity of diabetic subjects. J. Assoc. Physicians India, 2005, 53, 599-602.
[PMID: 16190127]
[6]
Southerland, J.H.; Taylor, G.W.; Offenbacher, S. Diabetes and periodontal infection:making the connection. Clin. Diabetes, 2005, 23(4), 171-178.
[http://dx.doi.org/10.2337/diaclin.23.4.171]
[7]
Webb, B.C.; Thomas, C.J.; Willcox, M.D.P.; Harty, D.W.S.; Knox, K.W. Candida-associated denture stomatitis. Aetiology and management: A review. Part 3. Treatment of oral candidosis. Aust. Dent. J., 1998, 43(3), 244-249.
[http://dx.doi.org/10.1111/j.1834-7819.1998.tb00172.x] [PMID: 9775471]
[8]
Budtz-Jørgensen, E.; Mojon, P.; Banon-Clément, J.M.; Baehni, P. Oral candidosis in long-term hospital care: Comparison of edentulous and dentate subjects. Oral Dis., 1996, 2(4), 285-290.
[http://dx.doi.org/10.1111/j.1601-0825.1996.tb00239.x] [PMID: 9171512]
[9]
Budtz-Jørgensen, E.; Mojon, P.; Rentsch, A.; Deslauriers, N. Effects of an oral health program on the occurrence of oral candidosis in a long-term care facility. Community Dent. Oral Epidemiol., 2000, 28(2), 141-149.
[http://dx.doi.org/10.1034/j.1600-0528.2000.028002141.x] [PMID: 10730723]
[10]
Heimdahl, A.; Nord, C.E. Oral yeast infections in immunocompromised and seriously diseased patients. Acta Odontol. Scand., 1990, 48(1), 77-84.
[http://dx.doi.org/10.3109/00016359009012737] [PMID: 2181814]
[11]
Holmstrup, P.; Axéll, T. Classification and clinical manifestations of oral yeast infections. Acta Odontol. Scand., 1990, 48(1), 57-59.
[http://dx.doi.org/10.3109/00016359009012734] [PMID: 2181811]
[12]
Arendorf, T.M.; Walker, D.M. Oral candidal populations in health and disease. Br. Dent. J., 1979, 147(10), 267-272.
[http://dx.doi.org/10.1038/sj.bdj.4804344] [PMID: 393283]
[13]
Schou, L.; Wight, C.; Cumming, C. Oral hygiene habits, denture plaque, presence of yeasts and stomatitis in institutionalized elderly in Lathian, Scotland. Community Dent. Oral Epidemiol., 1987, 15(2), 85-89.
[http://dx.doi.org/10.1111/j.1600-0528.1987.tb00488.x] [PMID: 3552396]
[14]
Wilkieson, C.; Samaranayake, L.P.; MacFarlane, T.W.; Lamey, P-J.; MacKenzie, D. Oral candidosis in the elderly in long term hospital care. J. Oral Pathol. Med., 1991, 20(1), 13-16.
[http://dx.doi.org/10.1111/j.1600-0714.1991.tb00880.x] [PMID: 1900531]
[15]
Könsberg, R.; Axéll, T. Treatment of Candida-infected denture stomatitis with a miconazole lacquer. Oral Surg. Oral Med. Oral Pathol., 1994, 78(3), 306-311.
[http://dx.doi.org/10.1016/0030-4220(94)90059-0] [PMID: 7970589]
[16]
Dhir, G.; Berzins, D.W.; Dhuru, V.B.; Periathamby, A.R.; Dentino, A. Physical properties of denture base resins potentially resistant to Candida adhesion. J. Prosthodont., 2007, 16(6), 465-472.
[http://dx.doi.org/10.1111/j.1532-849X.2007.00219.x] [PMID: 17672838]
[17]
Matsuura, T.; Abe, Y.; Sato, Y.; Okamoto, K.; Ueshige, M.; Akagawa, Y. Prolonged antimicrobial effect of tissue conditioners containing silver-zeolite. J. Dent., 1997, 25(5), 373-377.
[http://dx.doi.org/10.1016/S0300-5712(96)00050-4] [PMID: 9241955]
[18]
Casemiro, L.A.; Martins, C.H.G.; Pires-de-Souza, F.C.P.; Panzeri, H. Antimicrobial and mechanical properties of acrylic resins with incorporated silver-zinc zeolite - part I. Gerodontology, 2008, 25(3), 187-194.
[http://dx.doi.org/10.1111/j.1741-2358.2007.00198.x] [PMID: 18194331]
[19]
Brigger, I.; Dubernet, C.; Couvreur, P. Nanoparticles in cancer therapy and diagnosis. Adv. Drug Deliv. Rev., 2012, 64, 24-36.
[http://dx.doi.org/10.1016/j.addr.2012.09.006] [PMID: 12204596]
[20]
Gaffet, E.; Tachikart, M.; El Kedim, O.; Rahouadj, R. Nanostructural materials formation by mechanical alloying: Morphologic analysis based on transmission and scanning electron microscopic observations. Mater. Charact., 1996, 36(4-5), 185-190.
[http://dx.doi.org/10.1016/S1044-5803(96)00047-2]
[21]
Amulyavichus, A.; Daugvila, A.; Davidonis, R. Study of chemical composition of nanostructural materials prepared by laser cutting of metals. Fizika Metallov i Metallovedenie, 1998, 85(1), 111-117.
[22]
Tien, D.C.; Tseng, K.H.; Liao, C.Y.; Tsung, T.T. Colloidal silver fabrication using the spark discharge system and its antimicrobial effect on Staphylococcus aureus. Med. Eng. Phys., 2008, 30(8), 948-952.
[http://dx.doi.org/10.1016/j.medengphy.2007.10.007] [PMID: 18069039]
[23]
Bahrig, L.; Hickey, S.G.; Eychmüller, A. Mesocrystalline materials and the involvement of oriented attachment – a review. CrystEngComm, 2014, 16(40), 9408-9424.
[http://dx.doi.org/10.1039/C4CE00882K]
[24]
Mukherjee, P.; Ahmad, A.; Mandal, D.; Senapati, S.; Sainkar, S.R.; Khan, M.I.; Parishcha, R.; Ajaykumar, P.V.; Alam, M.; Kumar, R.; Sastry, M. Fungus-mediated synthesis of silver nanoparticles and their immobilization in the mycelial matrix: A novel biological approach to nanoparticle synthesis. Nano Lett., 2001, 1(10), 515-519.
[http://dx.doi.org/10.1021/nl0155274]
[25]
Vazquez-Muñoz, R.; Avalos-Borja, M.; Castro-Longoria, E. Ultrastructural analysis of Candida albicans when exposed to silver nanoparticles. PLoS One, 2014, 9(10)e108876
[http://dx.doi.org/10.1371/journal.pone.0108876] [PMID: 25290909]
[26]
Williams, D.F. The bio compatibility of silver. First International Conference on Gold and Silver in Medicine, 1987, pp. 261-272.
[27]
Kadir, T.; Pisiriciler, R.; Akyüz, S.; Yarat, A.; Emekli, N.; Ipbüker, A. Mycological and cytological examination of oral candidal carriage in diabetic patients and non-diabetic control subjects: Thorough analysis of local aetiologic and systemic factors. J. Oral Rehabil., 2002, 29(5), 452-457.
[http://dx.doi.org/10.1046/j.1365-2842.2002.00837.x] [PMID: 12028493]
[28]
Melo, N.R.; Taguchi, H.; Jorge, J.; Pedro, R.J.; Almeida, O.P.; Fukushima, K.; Nishimura, K.; Miyaji, M. Oral Candida flora from Brazilian human immunodeficiency virus-infected patients in the highly active antiretroviral therapy era. Mem. Inst. Oswaldo Cruz, 2004, 99(4), 425-431.
[http://dx.doi.org/10.1590/S0074-02762004000400014] [PMID: 15322634]
[29]
Lalla, R.V.; Patton, L.L. Dongari-BagtzoglouA.Oralcandidiasis:pathogenesis, clinical presentation, diagnosis and treatment strategies. J. Calif. Dent. Assoc., 2013, 41(4), 263-268.
[PMID: 23705242]
[30]
Lee, P.C.; Meisel, D. Adsorption and surface-enhanced Raman of dyes on silver and gold sols. J. Phys. Chem., 1982, 86(17), 3391-3395.
[http://dx.doi.org/10.1021/j100214a025]
[31]
Shirtcliffe, N.; Nickel, U.; Schneider, S. Reproducible preparation of silver sols with small particle size using borohydride reduction: For use as nuclei for preparation of larger particles. J. Colloid Interface Sci., 1999, 211(1), 122-129.
[http://dx.doi.org/10.1006/jcis.1998.5980] [PMID: 9929443]
[32]
Oliveira, M.M.; Ugarte, D.; Zanchet, D.; Zarbin, A.J.G. Influence of synthetic parameters on the size, structure, and stability of dodecanethiol-stabilized silver nanoparticles. J. Colloid Interface Sci., 2005, 292(2), 429-435.
[http://dx.doi.org/10.1016/j.jcis.2005.05.068] [PMID: 16055140]
[33]
Pradhan, N.; Pal, A.; Pal, T. Silver nanoparticle catalyzed reduction of aromatic nitro compounds. Colloids Surf. A Physicochem. Eng. Asp., 2002, 196(2-3), 247-257.
[http://dx.doi.org/10.1016/S0927-7757(01)01040-8]
[34]
Nersisyan, H.H.; Lee, J.H.; Son, H.T.; Won, C.W.; Maeng, D.Y. A new and effective chemical reduction method for preparation of nanosized silver powder and colloid dispersion. Mater. Res. Bull., 2003, 38(6), 949-956.
[http://dx.doi.org/10.1016/S0025-5408(03)00078-3]
[35]
Sondi, I.; Goia, D.V.; Matijević, E. Preparation of highly concentrated stable dispersions of uniform silver nanoparticles. J. Colloid Interface Sci., 2003, 260(1), 75-81.
[http://dx.doi.org/10.1016/S0021-9797(02)00205-9] [PMID: 12742036]
[36]
Feng, Q.L.; Wu, J.; Chen, G.Q.; Cui, F.Z.; Kim, T.N.; Kim, J.O. A mechanistic study of the antibacterial effect of silver ions onEscherichia coli andStaphylococcus aureus. J. Biomed. Mater. Res., 2000, 52(4), 662-668.
[http://dx.doi.org/10.1002/1097-4636(20001215)52:4<662:AID-JBM10>3.0.CO;2-3] [PMID: 11033548]
[37]
Spadaro, J.A.; Berger, T.J.; Barranco, S.D.; Chapin, S.E.; Becker, R.O. Antibacterial effects of silver electrodes with weak direct current. Antimicrob. Agents Chemother., 1974, 6(5), 637-642.
[http://dx.doi.org/10.1128/AAC.6.5.637] [PMID: 15825319]
[38]
Noginov, M.A.; Zhu, G.; Bahoura, M.; Adegoke, J.; Small, C.; Ritzo, B.A.; Drachev, V.P.; Shalaev, V.M. The effect of gain and absorption on surface plasmons in metal nanoparticles. Appl. Phys. B, 2007, 86(3), 455-460.
[http://dx.doi.org/10.1007/s00340-006-2401-0]
[39]
Link, S.; El-Sayed, M.A. Optical properties and ultrafast dynamics of metallic nanocrystals. Annu. Rev. Phys. Chem., 2003, 54(1), 331-366.
[http://dx.doi.org/10.1146/annurev.physchem.54.011002.103759] [PMID: 12626731]
[40]
Kumar, R.; Münstedt, H. Silver ion release from antimicrobial polyamide/silver composites. Biomaterials, 2005, 26(14), 2081-2088.
[http://dx.doi.org/10.1016/j.biomaterials.2004.05.030] [PMID: 15576182]
[41]
Saito, R.; Okamura, S.; Ishizu, K. Introduction of colloidal silver into poly(2-vinyl pyridine) microdomains of microphase separated poly(styrene-b-2-vinyl pyridine) film: 3. Poly(2-vinyl pyridine) spherical microdomain. Polymer, 1993, 34(6), 1189-1195.
[http://dx.doi.org/10.1016/0032-3861(93)90771-2]
[42]
Raszewski, Z. Dynamics of different ion release from denture-base acrylic resins and their mechanical properties after the addition of bioactive materials. Saudi Dent. J., 2021, 33(8), 1071-1077.
[http://dx.doi.org/10.1016/j.sdentj.2021.05.001] [PMID: 34916767]

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