β-lactamase and Plasmid-mediated Quinolone Resistance Determinants Among Proteus spp. Isolates at a Tertiary-care Hospital in Kolkata, India

Title:β-lactamase and Plasmid-mediated Quinolone Resistance Determinants Among Proteus spp. Isolates at a Tertiary-care Hospital in Kolkata, India

Volume: 22 Issue: 3

Author(s): Abhi Mallick, Mili Barik, Soma Sarkar and Surojit Das*

Affiliation:

• Department of Biomedical Laboratory Science and Management, Vidyasagar University, West Bengal, India

Keywords: Proteus spp., antimicrobial resistance, β-lactamase, plasmid-mediated quinolone resistance, acquired genes, human gut.

Abstract:

Background: Emerging antibiotic resistance (ABR) in Proteus spp., especially to third-generation cephalosporins (3GCc), carbapenems, and fluoroquinolones, challenges the treatment outcome and infection prevention. Limited studies pose a knowledge gap between them and ABR.

Methods: We investigated the in vitro efficacy of therapeutic options and prevalence of β-lactamase and plasmid-mediated quinolone resistance (PMQR) traits in 3GC- and/or fluoroquinolone- nonsusceptible Proteus (P.) spp. (n=27) in Kolkata, India, during 2021–2022. P. mirabilis was commonly isolated (>80%) from superficial and urine samples. The majority of the isolates (48-78%) remained susceptible to piperacillin-tazobactam, meropenem, amikacin, cefoperazonesulbactam, and cefepime.

Results: All isolates showed >0.2 multiple-antibiotic resistance index, with >65% being multidrug and >30% being extensively drug-resistant. bla_TEM (n=9), blaNDM (n=9), and qnrA (n=6) were commonly noted with the co-production of β-lactamases and PMQR in ten (37%) isolates. More than 50% of the isolates were devoid of the tested acquired genes.

Conclusion: The study concludes that superbugs dominate, with limited occurrence of plasmidborne markers in this geographic location.

Cite this article as:

Mallick Abhi, Barik Mili, Sarkar Soma and Das Surojit*, β-lactamase and Plasmid-mediated Quinolone Resistance Determinants Among Proteus spp. Isolates at a Tertiary-care Hospital in Kolkata, India, Anti-Infective Agents 2024; 22 (3) : e170124225724 . https://dx.doi.org/10.2174/0122113525279277231223035547