Application of Flow Cytometry in Biological Sciences

Flow cytometry is one of the sophisticated tools with its applications in different biological disciplines. It is potentially efficient in the characterization of mixed populations of cells present in biological samples, including blood cells, lymphocytes, microorganisms, sperms, cancer cells, metabolites, antibodies, DNA/RNA content, proteins, toxins, plant spores, etc. Flow cytometry is widely applied in the determination of cellular characteristics and cellular components profiling like cell size, intracellular pH, DNA, RNA, proteins, surface receptors, membrane potential, calcium, and others. Currently, flow cytometry is pragmatic in basic as well as applied plant research and plant industrial applications like plant breeding. Flow cytometry has been considered a reliable, rapid, efficient, and accurate tool for analysis of ploidy level and nuclear genome size estimation. It is also subjected to taxonomy to study population/subpopulation dynamics. Gender determination from pollen grain is also possible due to flow cytometry. 

Keywords: Apoptosis, Bioprocesses, Cell sorting, Cell viability, Caspase-3, Cytomegalovirus, Cell Cycle, Endoreduplication, Flow cytometry, Genome size estimation, Hematologic Malignancy, HLA antibody profiling, Intraspecific variation, Immunophenotyping, IPEX, In vivo compartmentalization, LRBA, Ploidy determination, Primary immunodeficiency, Plant breeding, Sorting pollen, Taxonomy, X- linked agammaglobulinemia, Uncultivated diversity, Wiskott-Aldrich syndrome.