Abstract
This review introduces the concept of enzyme biosensors. As the major feature of an enzyme is its selectivity and specificity, we briefly highlighted the principles of enzyme production and enzyme classification. Moreover, enzyme immobilization methods, including physical adsorption and entrapment techniques with special attention on cross-linking and covalent binding are discussed followed by different electrochemical methods used in the biosensors fabrication. Furthermore, a comprehensive discussion about horseradish peroxidase (HRP) as a model enzyme is presented with investigation of some modified enzyme electrodes such as screen-printed and carbon paste electrodes. Some approaches to enhance biosensors performances and their applications are also highlighted.
Keywords: Biosensors, enzyme, electrochemistry, immobilization techniques, modified electrodes.
Current Analytical Chemistry
Title:Recent Advances on Electrochemical Enzyme Biosensors
Volume: 12 Issue: 1
Author(s): Ezzaldeen Younes Jomma and Shou-Nian Ding
Affiliation:
Keywords: Biosensors, enzyme, electrochemistry, immobilization techniques, modified electrodes.
Abstract: This review introduces the concept of enzyme biosensors. As the major feature of an enzyme is its selectivity and specificity, we briefly highlighted the principles of enzyme production and enzyme classification. Moreover, enzyme immobilization methods, including physical adsorption and entrapment techniques with special attention on cross-linking and covalent binding are discussed followed by different electrochemical methods used in the biosensors fabrication. Furthermore, a comprehensive discussion about horseradish peroxidase (HRP) as a model enzyme is presented with investigation of some modified enzyme electrodes such as screen-printed and carbon paste electrodes. Some approaches to enhance biosensors performances and their applications are also highlighted.
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Cite this article as:
Jomma Younes Ezzaldeen and Ding Shou-Nian, Recent Advances on Electrochemical Enzyme Biosensors, Current Analytical Chemistry 2016; 12 (1) . https://dx.doi.org/10.2174/1573411011666150611184215
DOI https://dx.doi.org/10.2174/1573411011666150611184215 |
Print ISSN 1573-4110 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-6727 |
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