Abstract
Proteins participate in almost every cell physiological function, and to do so, they need to reach a state that allows its function by folding and/or exposing surfaces of interactions. Spontaneous folding in the cell is in general hindered by its crowded and viscous environment, which favors misfolding and nonspecific and deleterious self-interactions. To overcome this, cells have a system, in which Hsp70 and Hsp90 play a central role to aid protein folding and avoid misfolding. The topics of this review include the biophysical tools used for monitoring protein-ligand and protein-protein interactions and also some important results related to the study of molecular chaperones and heat shock proteins (Hsp), with a focus on the Hsp70/Hsp90 network. The biophysical tools and their use to probe the conformation and interaction of Hsp70 and Hsp90 are briefly reviewed.
Keywords: Analytical ultracentrifugation, Calorimetry, Fluorescence, Molecular chaperones and Hsps, Protein folding, Protein interaction, Thermodynamics.
Current Protein & Peptide Science
Title:From Conformation to Interaction: Techniques to Explore the Hsp70/ Hsp90 Network
Volume: 16 Issue: 8
Author(s): Fernanda A.H. Batista, Lisandra M. Gava, Glaucia M. S. Pinheiro, Carlos H.I. Ramos and Julio C. Borges
Affiliation:
Keywords: Analytical ultracentrifugation, Calorimetry, Fluorescence, Molecular chaperones and Hsps, Protein folding, Protein interaction, Thermodynamics.
Abstract: Proteins participate in almost every cell physiological function, and to do so, they need to reach a state that allows its function by folding and/or exposing surfaces of interactions. Spontaneous folding in the cell is in general hindered by its crowded and viscous environment, which favors misfolding and nonspecific and deleterious self-interactions. To overcome this, cells have a system, in which Hsp70 and Hsp90 play a central role to aid protein folding and avoid misfolding. The topics of this review include the biophysical tools used for monitoring protein-ligand and protein-protein interactions and also some important results related to the study of molecular chaperones and heat shock proteins (Hsp), with a focus on the Hsp70/Hsp90 network. The biophysical tools and their use to probe the conformation and interaction of Hsp70 and Hsp90 are briefly reviewed.
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Cite this article as:
Batista A.H. Fernanda, Gava M. Lisandra, Pinheiro M. S. Glaucia, Ramos H.I. Carlos and Borges C. Julio, From Conformation to Interaction: Techniques to Explore the Hsp70/ Hsp90 Network, Current Protein & Peptide Science 2015; 16 (8) . https://dx.doi.org/10.2174/1389203716666150505225744
DOI https://dx.doi.org/10.2174/1389203716666150505225744 |
Print ISSN 1389-2037 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5550 |
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