Abstract
Site-directed mutagenesis allows elucidation of the basic principles of the porin-driven membrane permeability and opens the possibility for the modulation of functional states of porin channels. The review is aimed to show the advantages of using mutant and chemically modified porins for obtaining detailed information about molecular mechanisms that underlie the non-specific transmembrane diffusion. We summarized data regarding the effects of the point substitutions and the external loop deletions on electrophysiological properties of general porins. The influence of charges inside the pore eyelet and the roles of external loops in ion conductance, ion selectivity, and voltage gating were described.
Keywords: Outer membrane porins, electrophysiological properties, site-directed mutagenesis, point substitutions and loop deletions, pore selectivity, voltage gating.
Current Protein & Peptide Science
Title:Modified and Mutant Porins in the Study on Molecular Basis of Non- Specific Diffusion
Volume: 18 Issue: 3
Author(s): Olga D. Novikova, Olga Yu. Portnyagina and Tamara F. Solov’eva
Affiliation:
Keywords: Outer membrane porins, electrophysiological properties, site-directed mutagenesis, point substitutions and loop deletions, pore selectivity, voltage gating.
Abstract: Site-directed mutagenesis allows elucidation of the basic principles of the porin-driven membrane permeability and opens the possibility for the modulation of functional states of porin channels. The review is aimed to show the advantages of using mutant and chemically modified porins for obtaining detailed information about molecular mechanisms that underlie the non-specific transmembrane diffusion. We summarized data regarding the effects of the point substitutions and the external loop deletions on electrophysiological properties of general porins. The influence of charges inside the pore eyelet and the roles of external loops in ion conductance, ion selectivity, and voltage gating were described.
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Cite this article as:
Novikova D. Olga, Portnyagina Yu. Olga and Solov’eva F. Tamara, Modified and Mutant Porins in the Study on Molecular Basis of Non- Specific Diffusion, Current Protein & Peptide Science 2017; 18 (3) . https://dx.doi.org/10.2174/1389203717666160905145514
DOI https://dx.doi.org/10.2174/1389203717666160905145514 |
Print ISSN 1389-2037 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5550 |
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